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An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria

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An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria. / Porter, J.; Robinson, J.; Pickup, R. et al.
In: Journal of Applied Microbiology, Vol. 84, No. 5, 01.06.1998, p. 722-732.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Porter, J, Robinson, J, Pickup, R & Edwards, C 1998, 'An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria', Journal of Applied Microbiology, vol. 84, no. 5, pp. 722-732. https://doi.org/10.1046/j.1365-2672.1998.00403.x

APA

Porter, J., Robinson, J., Pickup, R., & Edwards, C. (1998). An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria. Journal of Applied Microbiology, 84(5), 722-732. https://doi.org/10.1046/j.1365-2672.1998.00403.x

Vancouver

Porter J, Robinson J, Pickup R, Edwards C. An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria. Journal of Applied Microbiology. 1998 Jun 1;84(5):722-732. doi: 10.1046/j.1365-2672.1998.00403.x

Author

Porter, J. ; Robinson, J. ; Pickup, R. et al. / An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria. In: Journal of Applied Microbiology. 1998 ; Vol. 84, No. 5. pp. 722-732.

Bibtex

@article{88127b479993478d8e8fad82c4ee085a,
title = "An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria",
abstract = "Lectin-labelled magnetic beads were assessed and compared with antisera as an alternative approach for the targeted separation and isolation of enteric bacteria. Of the 16 lectins tested against a range of bacterial species, concanavalin A (conA) showed the greatest potential. Agglutination of bacterial cells in suspension using conA and methods for effective labelling of the magnetic beads with the lectin were optimized. ConA-labelled magnetic beads were compared with antibody-labelled beads for recovery of bacterial cells from pure or mixed laboratory cultures and from natural populations in river water. Recovered cell populations were free from environmental impurities and a high percentage of the culturable cells was extracted. Specific cell recovery was found to be variable, but the use of lectins offers some promise as an alternative cell discriminator.",
author = "J. Porter and J. Robinson and R. Pickup and C. Edwards",
year = "1998",
month = jun,
day = "1",
doi = "10.1046/j.1365-2672.1998.00403.x",
language = "English",
volume = "84",
pages = "722--732",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria

AU - Porter, J.

AU - Robinson, J.

AU - Pickup, R.

AU - Edwards, C.

PY - 1998/6/1

Y1 - 1998/6/1

N2 - Lectin-labelled magnetic beads were assessed and compared with antisera as an alternative approach for the targeted separation and isolation of enteric bacteria. Of the 16 lectins tested against a range of bacterial species, concanavalin A (conA) showed the greatest potential. Agglutination of bacterial cells in suspension using conA and methods for effective labelling of the magnetic beads with the lectin were optimized. ConA-labelled magnetic beads were compared with antibody-labelled beads for recovery of bacterial cells from pure or mixed laboratory cultures and from natural populations in river water. Recovered cell populations were free from environmental impurities and a high percentage of the culturable cells was extracted. Specific cell recovery was found to be variable, but the use of lectins offers some promise as an alternative cell discriminator.

AB - Lectin-labelled magnetic beads were assessed and compared with antisera as an alternative approach for the targeted separation and isolation of enteric bacteria. Of the 16 lectins tested against a range of bacterial species, concanavalin A (conA) showed the greatest potential. Agglutination of bacterial cells in suspension using conA and methods for effective labelling of the magnetic beads with the lectin were optimized. ConA-labelled magnetic beads were compared with antibody-labelled beads for recovery of bacterial cells from pure or mixed laboratory cultures and from natural populations in river water. Recovered cell populations were free from environmental impurities and a high percentage of the culturable cells was extracted. Specific cell recovery was found to be variable, but the use of lectins offers some promise as an alternative cell discriminator.

U2 - 10.1046/j.1365-2672.1998.00403.x

DO - 10.1046/j.1365-2672.1998.00403.x

M3 - Journal article

C2 - 9674124

AN - SCOPUS:0031748922

VL - 84

SP - 722

EP - 732

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 5

ER -