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Analysis of amino acids by miniaturised isotachophoresis

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<mark>Journal publication date</mark>8/10/2004
<mark>Journal</mark>Journal of Chromatography A
Issue number1-2
Volume1051
Number of pages6
Pages (from-to)221-226
Publication StatusPublished
Early online date15/06/04
<mark>Original language</mark>English

Abstract

A method allowing the miniaturised isotachophoretic analysis of amino acids has been developed. To overcome the problems of carbonate contamination which occur when performing separations at alkaline pH levels glycolate was used as the leading ion. Addition of magnesium to the leading electrolyte as a counter species was found to improve the separations. The method has been used on a poly(methyl methacrylate) microdevice with integrated on-column conductivity detectors. The behaviour of a range of common amino acids was investigated and successful separations of up to seven amino acids were made. Good linearity was observed with calibration curves for aspartic acid and phenylalanine over the range 0.063–1.0 mM. Limits of detection for these two species were calculated to be 0.060 and 0.018 mM, respectively.