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Analysis of amino acids by miniaturised isotachophoresis

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Analysis of amino acids by miniaturised isotachophoresis. / Prest, Jeff E.; Baldock, Sara J.; Fielden, Peter R. et al.
In: Journal of Chromatography A, Vol. 1051, No. 1-2, 08.10.2004, p. 221-226.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Prest, JE, Baldock, SJ, Fielden, PR, Goddard, NJ & Treves Brown, BJ 2004, 'Analysis of amino acids by miniaturised isotachophoresis', Journal of Chromatography A, vol. 1051, no. 1-2, pp. 221-226. https://doi.org/10.1016/j.chroma.2004.05.026

APA

Prest, J. E., Baldock, S. J., Fielden, P. R., Goddard, N. J., & Treves Brown, B. J. (2004). Analysis of amino acids by miniaturised isotachophoresis. Journal of Chromatography A, 1051(1-2), 221-226. https://doi.org/10.1016/j.chroma.2004.05.026

Vancouver

Prest JE, Baldock SJ, Fielden PR, Goddard NJ, Treves Brown BJ. Analysis of amino acids by miniaturised isotachophoresis. Journal of Chromatography A. 2004 Oct 8;1051(1-2):221-226. Epub 2004 Jun 15. doi: 10.1016/j.chroma.2004.05.026

Author

Prest, Jeff E. ; Baldock, Sara J. ; Fielden, Peter R. et al. / Analysis of amino acids by miniaturised isotachophoresis. In: Journal of Chromatography A. 2004 ; Vol. 1051, No. 1-2. pp. 221-226.

Bibtex

@article{20c9aa25c5704d70a0f8ed9a667cd707,
title = "Analysis of amino acids by miniaturised isotachophoresis",
abstract = "A method allowing the miniaturised isotachophoretic analysis of amino acids has been developed. To overcome the problems of carbonate contamination which occur when performing separations at alkaline pH levels glycolate was used as the leading ion. Addition of magnesium to the leading electrolyte as a counter species was found to improve the separations. The method has been used on a poly(methyl methacrylate) microdevice with integrated on-column conductivity detectors. The behaviour of a range of common amino acids was investigated and successful separations of up to seven amino acids were made. Good linearity was observed with calibration curves for aspartic acid and phenylalanine over the range 0.063–1.0 mM. Limits of detection for these two species were calculated to be 0.060 and 0.018 mM, respectively.",
keywords = "Isotachophoresis, Chip technology, Instrumentation, Miniaturisation, Amino acids",
author = "Prest, {Jeff E.} and Baldock, {Sara J.} and Fielden, {Peter R.} and Goddard, {Nicholas J.} and {Treves Brown}, {Bernard J.}",
year = "2004",
month = oct,
day = "8",
doi = "10.1016/j.chroma.2004.05.026",
language = "English",
volume = "1051",
pages = "221--226",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Analysis of amino acids by miniaturised isotachophoresis

AU - Prest, Jeff E.

AU - Baldock, Sara J.

AU - Fielden, Peter R.

AU - Goddard, Nicholas J.

AU - Treves Brown, Bernard J.

PY - 2004/10/8

Y1 - 2004/10/8

N2 - A method allowing the miniaturised isotachophoretic analysis of amino acids has been developed. To overcome the problems of carbonate contamination which occur when performing separations at alkaline pH levels glycolate was used as the leading ion. Addition of magnesium to the leading electrolyte as a counter species was found to improve the separations. The method has been used on a poly(methyl methacrylate) microdevice with integrated on-column conductivity detectors. The behaviour of a range of common amino acids was investigated and successful separations of up to seven amino acids were made. Good linearity was observed with calibration curves for aspartic acid and phenylalanine over the range 0.063–1.0 mM. Limits of detection for these two species were calculated to be 0.060 and 0.018 mM, respectively.

AB - A method allowing the miniaturised isotachophoretic analysis of amino acids has been developed. To overcome the problems of carbonate contamination which occur when performing separations at alkaline pH levels glycolate was used as the leading ion. Addition of magnesium to the leading electrolyte as a counter species was found to improve the separations. The method has been used on a poly(methyl methacrylate) microdevice with integrated on-column conductivity detectors. The behaviour of a range of common amino acids was investigated and successful separations of up to seven amino acids were made. Good linearity was observed with calibration curves for aspartic acid and phenylalanine over the range 0.063–1.0 mM. Limits of detection for these two species were calculated to be 0.060 and 0.018 mM, respectively.

KW - Isotachophoresis

KW - Chip technology

KW - Instrumentation

KW - Miniaturisation

KW - Amino acids

U2 - 10.1016/j.chroma.2004.05.026

DO - 10.1016/j.chroma.2004.05.026

M3 - Journal article

VL - 1051

SP - 221

EP - 226

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -