Home > Research > Publications & Outputs > Analysis of pyomelanin formation
View graph of relations

Analysis of pyomelanin formation

Research output: Contribution to conference - Without ISBN/ISSN Posterpeer-review

Published

Standard

Analysis of pyomelanin formation. / Galeb, Hanaa; Hardy, John; Taylor, Adam.
2021. Poster session presented at Analytical Research Forum 2021.

Research output: Contribution to conference - Without ISBN/ISSN Posterpeer-review

Harvard

Galeb, H, Hardy, J & Taylor, A 2021, 'Analysis of pyomelanin formation', Analytical Research Forum 2021, 15/06/21 - 16/06/21.

APA

Galeb, H., Hardy, J., & Taylor, A. (2021). Analysis of pyomelanin formation. Poster session presented at Analytical Research Forum 2021.

Vancouver

Galeb H, Hardy J, Taylor A. Analysis of pyomelanin formation. 2021. Poster session presented at Analytical Research Forum 2021.

Author

Galeb, Hanaa ; Hardy, John ; Taylor, Adam. / Analysis of pyomelanin formation. Poster session presented at Analytical Research Forum 2021.

Bibtex

@conference{b082e586155b447686628a195c9405b7,
title = "Analysis of pyomelanin formation",
abstract = "The accumulation of homogentisic acid (HGA) in patients with alkaptonuria is associated with the concomitant deposition of pigments contain significant amounts of polymerised HGA (polyHGA) in the bodily tissues of the patients. The polymerisation of HGA under various different conditions in vitro was investigated to attempt to understand if there is a correlation between the conditions and rate of pigment deposition in vivo. In this study, we applied a selection of different analytical chemistry techniques including: nuclear magnetic resonance spectroscopy (NMR), high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX), UV-visible spectroscopy (UV-visible) and electron paramagnetic resonance (EPR). We observed condition-dependent polyHGA formation, and are currently correlating these findings with literature data (e.g. histological studies) to understand polyHGA pigment deposition inside specific tissues in the body.",
author = "Hanaa Galeb and John Hardy and Adam Taylor",
year = "2021",
month = jun,
day = "15",
language = "English",
note = "Analytical Research Forum 2021, ARF21 ; Conference date: 15-06-2021 Through 16-06-2021",
url = "https://www.rsc.org/events/detail/46233/analytical-research-forum-2021-arf21",

}

RIS

TY - CONF

T1 - Analysis of pyomelanin formation

AU - Galeb, Hanaa

AU - Hardy, John

AU - Taylor, Adam

PY - 2021/6/15

Y1 - 2021/6/15

N2 - The accumulation of homogentisic acid (HGA) in patients with alkaptonuria is associated with the concomitant deposition of pigments contain significant amounts of polymerised HGA (polyHGA) in the bodily tissues of the patients. The polymerisation of HGA under various different conditions in vitro was investigated to attempt to understand if there is a correlation between the conditions and rate of pigment deposition in vivo. In this study, we applied a selection of different analytical chemistry techniques including: nuclear magnetic resonance spectroscopy (NMR), high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX), UV-visible spectroscopy (UV-visible) and electron paramagnetic resonance (EPR). We observed condition-dependent polyHGA formation, and are currently correlating these findings with literature data (e.g. histological studies) to understand polyHGA pigment deposition inside specific tissues in the body.

AB - The accumulation of homogentisic acid (HGA) in patients with alkaptonuria is associated with the concomitant deposition of pigments contain significant amounts of polymerised HGA (polyHGA) in the bodily tissues of the patients. The polymerisation of HGA under various different conditions in vitro was investigated to attempt to understand if there is a correlation between the conditions and rate of pigment deposition in vivo. In this study, we applied a selection of different analytical chemistry techniques including: nuclear magnetic resonance spectroscopy (NMR), high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX), UV-visible spectroscopy (UV-visible) and electron paramagnetic resonance (EPR). We observed condition-dependent polyHGA formation, and are currently correlating these findings with literature data (e.g. histological studies) to understand polyHGA pigment deposition inside specific tissues in the body.

M3 - Poster

T2 - Analytical Research Forum 2021

Y2 - 15 June 2021 through 16 June 2021

ER -