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Application of two-dimensional correlation analysis to Raman optical activity

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<mark>Journal publication date</mark>6/11/2006
<mark>Journal</mark>Journal of Molecular Structure
Issue number1-3
Volume799
Number of pages11
Pages (from-to)61-71
Publication StatusPublished
<mark>Original language</mark>English
Event3rd International Symposium on Two-Dimensional Correlation Spectroscopy (2DCOS-3) - Delavan, United Kingdom
Duration: 12/08/200514/08/2005

Conference

Conference3rd International Symposium on Two-Dimensional Correlation Spectroscopy (2DCOS-3)
CountryUnited Kingdom
Period12/08/0514/08/05

Abstract

Raman optical activity (ROA) is an incisive probe of structural dynamics and its combination with two-dimensional correlation analysis is an attractive method for the investigation of conformational transitions in proteins. In this paper we show that pretreatment of data is essential for the generation of reliable 2D ROA and 2D Raman correlations. Specific experimental parameters and available information of ROA and Raman studies have facilitated the development of four pretreatment processes, baseline subtraction, smoothing, normalization and further baseline correction. The bisignate nature of ROA spectra has a significant effect on the interpretation of synchronous 2D correlation plots when bands at wavenumbers of both positive and negative intensity are compared, while interpretation of asynchronous plots remains unchanged. We introduce a notation format to make interpretation of 2D correlations from bisignate spectra clearer. With appropriate application of 2D correlation analysis to ROA spectra, 2D ROA and 2D Raman/ROA heterocorrelation are potentially valuable tools for the analysis of conformational transitions in polypeptides and proteins. (c) 2006 Elsevier B.V. All rights reserved.