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Application of two-dimensional correlation analysis to Raman optical activity

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Application of two-dimensional correlation analysis to Raman optical activity. / Ashton, Lorna; Czarnik-Matusewicz, Boguslawa; Blanch, Ewan W.
In: Journal of Molecular Structure, Vol. 799, No. 1-3, 06.11.2006, p. 61-71.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ashton, L, Czarnik-Matusewicz, B & Blanch, EW 2006, 'Application of two-dimensional correlation analysis to Raman optical activity', Journal of Molecular Structure, vol. 799, no. 1-3, pp. 61-71. https://doi.org/10.1016/j.molstruc.2006.03.042

APA

Ashton, L., Czarnik-Matusewicz, B., & Blanch, E. W. (2006). Application of two-dimensional correlation analysis to Raman optical activity. Journal of Molecular Structure, 799(1-3), 61-71. https://doi.org/10.1016/j.molstruc.2006.03.042

Vancouver

Ashton L, Czarnik-Matusewicz B, Blanch EW. Application of two-dimensional correlation analysis to Raman optical activity. Journal of Molecular Structure. 2006 Nov 6;799(1-3):61-71. doi: 10.1016/j.molstruc.2006.03.042

Author

Ashton, Lorna ; Czarnik-Matusewicz, Boguslawa ; Blanch, Ewan W. / Application of two-dimensional correlation analysis to Raman optical activity. In: Journal of Molecular Structure. 2006 ; Vol. 799, No. 1-3. pp. 61-71.

Bibtex

@article{8715d44a2eb343378d45d841b523bcbb,
title = "Application of two-dimensional correlation analysis to Raman optical activity",
abstract = "Raman optical activity (ROA) is an incisive probe of structural dynamics and its combination with two-dimensional correlation analysis is an attractive method for the investigation of conformational transitions in proteins. In this paper we show that pretreatment of data is essential for the generation of reliable 2D ROA and 2D Raman correlations. Specific experimental parameters and available information of ROA and Raman studies have facilitated the development of four pretreatment processes, baseline subtraction, smoothing, normalization and further baseline correction. The bisignate nature of ROA spectra has a significant effect on the interpretation of synchronous 2D correlation plots when bands at wavenumbers of both positive and negative intensity are compared, while interpretation of asynchronous plots remains unchanged. We introduce a notation format to make interpretation of 2D correlations from bisignate spectra clearer. With appropriate application of 2D correlation analysis to ROA spectra, 2D ROA and 2D Raman/ROA heterocorrelation are potentially valuable tools for the analysis of conformational transitions in polypeptides and proteins. (c) 2006 Elsevier B.V. All rights reserved.",
keywords = "Raman optical activity, two-dimensional correlation analysis, polylysine, conformational transition, normalization, bisignate, DEPENDENT SPECTRAL VARIATIONS, INFRARED CORRELATION SPECTROSCOPY, IR CORRELATION SPECTROSCOPY, SECONDARY STRUCTURE, CIRCULAR-DICHROISM, DYNAMIC SPECTRA, STATIC MODIFICATION, BETA-LACTOGLOBULIN, FT-IR, NORMALIZATION",
author = "Lorna Ashton and Boguslawa Czarnik-Matusewicz and Blanch, {Ewan W.}",
year = "2006",
month = nov,
day = "6",
doi = "10.1016/j.molstruc.2006.03.042",
language = "English",
volume = "799",
pages = "61--71",
journal = "Journal of Molecular Structure",
issn = "0022-2860",
publisher = "Elsevier",
number = "1-3",
note = "3rd International Symposium on Two-Dimensional Correlation Spectroscopy (2DCOS-3) ; Conference date: 12-08-2005 Through 14-08-2005",

}

RIS

TY - JOUR

T1 - Application of two-dimensional correlation analysis to Raman optical activity

AU - Ashton, Lorna

AU - Czarnik-Matusewicz, Boguslawa

AU - Blanch, Ewan W.

PY - 2006/11/6

Y1 - 2006/11/6

N2 - Raman optical activity (ROA) is an incisive probe of structural dynamics and its combination with two-dimensional correlation analysis is an attractive method for the investigation of conformational transitions in proteins. In this paper we show that pretreatment of data is essential for the generation of reliable 2D ROA and 2D Raman correlations. Specific experimental parameters and available information of ROA and Raman studies have facilitated the development of four pretreatment processes, baseline subtraction, smoothing, normalization and further baseline correction. The bisignate nature of ROA spectra has a significant effect on the interpretation of synchronous 2D correlation plots when bands at wavenumbers of both positive and negative intensity are compared, while interpretation of asynchronous plots remains unchanged. We introduce a notation format to make interpretation of 2D correlations from bisignate spectra clearer. With appropriate application of 2D correlation analysis to ROA spectra, 2D ROA and 2D Raman/ROA heterocorrelation are potentially valuable tools for the analysis of conformational transitions in polypeptides and proteins. (c) 2006 Elsevier B.V. All rights reserved.

AB - Raman optical activity (ROA) is an incisive probe of structural dynamics and its combination with two-dimensional correlation analysis is an attractive method for the investigation of conformational transitions in proteins. In this paper we show that pretreatment of data is essential for the generation of reliable 2D ROA and 2D Raman correlations. Specific experimental parameters and available information of ROA and Raman studies have facilitated the development of four pretreatment processes, baseline subtraction, smoothing, normalization and further baseline correction. The bisignate nature of ROA spectra has a significant effect on the interpretation of synchronous 2D correlation plots when bands at wavenumbers of both positive and negative intensity are compared, while interpretation of asynchronous plots remains unchanged. We introduce a notation format to make interpretation of 2D correlations from bisignate spectra clearer. With appropriate application of 2D correlation analysis to ROA spectra, 2D ROA and 2D Raman/ROA heterocorrelation are potentially valuable tools for the analysis of conformational transitions in polypeptides and proteins. (c) 2006 Elsevier B.V. All rights reserved.

KW - Raman optical activity

KW - two-dimensional correlation analysis

KW - polylysine

KW - conformational transition

KW - normalization

KW - bisignate

KW - DEPENDENT SPECTRAL VARIATIONS

KW - INFRARED CORRELATION SPECTROSCOPY

KW - IR CORRELATION SPECTROSCOPY

KW - SECONDARY STRUCTURE

KW - CIRCULAR-DICHROISM

KW - DYNAMIC SPECTRA

KW - STATIC MODIFICATION

KW - BETA-LACTOGLOBULIN

KW - FT-IR

KW - NORMALIZATION

U2 - 10.1016/j.molstruc.2006.03.042

DO - 10.1016/j.molstruc.2006.03.042

M3 - Journal article

VL - 799

SP - 61

EP - 71

JO - Journal of Molecular Structure

JF - Journal of Molecular Structure

SN - 0022-2860

IS - 1-3

T2 - 3rd International Symposium on Two-Dimensional Correlation Spectroscopy (2DCOS-3)

Y2 - 12 August 2005 through 14 August 2005

ER -