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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Application of vibrational spectroscopy techniques to non-destructively monitor plant health and development
AU - Butler, Holly
AU - McAinsh, Martin
AU - Adams, Steven
AU - Martin, Frank
N1 - This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
PY - 2015/5/21
Y1 - 2015/5/21
N2 - ibrational spectroscopy is a powerful analytical tool that is yet to be fully developed in plant science. Previously, such tools have been primarily applied to fixed or in vitro biological materials, which do not effectively encapsulate real-time physiological conditions of whole organisms. Coupled with multivariate analysis, this study examines the potential application of ATR-FTIR or Raman spectroscopy to determine spectral alterations indicative of healthy plant growth in leaf samples of Solanum lycopersicum. This was achieved in the absence of destructive effects on leaf tissues locally or on plant health systemically; additionally, autofluorescence was not a confounder. Feature extraction techniques including PCA-LDA were employed to examine variance within spectral datasets. In vivo measurements are able to successfully characterise key constituents of the leaf cuticle and cell wall, whilst qualifying leaf growth. Major alterations in carbohydrate and protein content of leaves were observed, correlating with known processes within leaf development from cell wall expansion to leaf senescence. These findings show that vibrational spectroscopy is an ideal technique for in vivo investigations in plant tissues.
AB - ibrational spectroscopy is a powerful analytical tool that is yet to be fully developed in plant science. Previously, such tools have been primarily applied to fixed or in vitro biological materials, which do not effectively encapsulate real-time physiological conditions of whole organisms. Coupled with multivariate analysis, this study examines the potential application of ATR-FTIR or Raman spectroscopy to determine spectral alterations indicative of healthy plant growth in leaf samples of Solanum lycopersicum. This was achieved in the absence of destructive effects on leaf tissues locally or on plant health systemically; additionally, autofluorescence was not a confounder. Feature extraction techniques including PCA-LDA were employed to examine variance within spectral datasets. In vivo measurements are able to successfully characterise key constituents of the leaf cuticle and cell wall, whilst qualifying leaf growth. Major alterations in carbohydrate and protein content of leaves were observed, correlating with known processes within leaf development from cell wall expansion to leaf senescence. These findings show that vibrational spectroscopy is an ideal technique for in vivo investigations in plant tissues.
U2 - 10.1039/C5AY00377F
DO - 10.1039/C5AY00377F
M3 - Journal article
VL - 7
SP - 4059
EP - 4070
JO - Analytical Methods
JF - Analytical Methods
SN - 1759-9660
IS - 10
ER -