Bioactive nano-hydroxyapatites have been synthesised for orbital floor repair and regeneration. Hydroxyapatite (HA) is widely used for bone repair and regeneration. It is composed of multiple anionic and cationic species, such as
carbonate, fluoride, phosphate, sodium, magnesium, silicon and citrate. However, the development of bioactive materials that can repair and regenerate bone is crucial for orbital floor fracture repair. Different ionic-substituted hydroxyapatites that included carbonate, fluoride and citrate were prepared by using a low-temperature hydrothermal flow process and their chemical and physical properties evaluated. Biological properties were evaluated by analysing cell viability of these synthesised materials by Alamar Blue cell metabolic activity assay with two different cell lines (MG63 and HTERT-BMSC’s). Results confirmed that ionic substitution with fluoride and citrate improved biocompatibility and
cell viability of synthesised hydroxyapatites.