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Biological and genotypic characterization of the Newcastle disease virus isolated from disease outbreaks in commercial poultry farms in northern Punjab, Pakistan

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Biological and genotypic characterization of the Newcastle disease virus isolated from disease outbreaks in commercial poultry farms in northern Punjab, Pakistan. / Farooq, Muhammad; Saliha, Uneeb; Munir, Muhammad et al.
In: Virology Reports, Vol. 3-4, 01.09.2014, p. 30-39.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Farooq, M, Saliha, U, Munir, M & Khan, QM 2014, 'Biological and genotypic characterization of the Newcastle disease virus isolated from disease outbreaks in commercial poultry farms in northern Punjab, Pakistan', Virology Reports, vol. 3-4, pp. 30-39. https://doi.org/10.1016/j.virep.2014.10.002

APA

Farooq, M., Saliha, U., Munir, M., & Khan, Q. M. (2014). Biological and genotypic characterization of the Newcastle disease virus isolated from disease outbreaks in commercial poultry farms in northern Punjab, Pakistan. Virology Reports, 3-4, 30-39. https://doi.org/10.1016/j.virep.2014.10.002

Vancouver

Farooq M, Saliha U, Munir M, Khan QM. Biological and genotypic characterization of the Newcastle disease virus isolated from disease outbreaks in commercial poultry farms in northern Punjab, Pakistan. Virology Reports. 2014 Sept 1;3-4:30-39. doi: 10.1016/j.virep.2014.10.002

Author

Farooq, Muhammad ; Saliha, Uneeb ; Munir, Muhammad et al. / Biological and genotypic characterization of the Newcastle disease virus isolated from disease outbreaks in commercial poultry farms in northern Punjab, Pakistan. In: Virology Reports. 2014 ; Vol. 3-4. pp. 30-39.

Bibtex

@article{900bfea12f374ee6925200313927811d,
title = "Biological and genotypic characterization of the Newcastle disease virus isolated from disease outbreaks in commercial poultry farms in northern Punjab, Pakistan",
abstract = "Newcastle disease (ND) is a highly contagious disease of many avian species and is particularly responsible for devastating disease outbreaks in commercial poultry flocks in Pakistan that incur huge economic losses to the national poultry industry annually. Despite implementation of an extensive vaccination program for poultry birds, the disease appears in an endemic form in commercial broiler and layer poultry farms. This study was conducted to identify the prevalent velogenic NDV strain responsible for disease outbreaks in commercial poultry farms in Punjab, Pakistan. The NDV strains isolated from pathological specimens through inoculation in embryonated chicken eggs were characterized biologically through the intracerebral pathogenicity index (ICPI), and genetically on the basis of the fusion (F) protein cleavage site. Among these, six NDV isolates showed an F protein cleavage site motif (112RRQKRF117) and an ICPI value ranging between 1.5 and 1.88, both are characteristics for velogenic strains of NDV. In addition, phylogenetic analysis based on a partial sequence of the F protein gene clustered these isolates within class II, genotype VII and specifically within genotype VII-e. This is the first report that demonstrated the presence of such NDV strains in commercial poultry farms in northern Punjab of Pakistan.",
keywords = "Newcastle disease, Pakistan, Pathotyping, Phylogenetic analysis, Poultry, Sequencing",
author = "Muhammad Farooq and Uneeb Saliha and Muhammad Munir and Khan, {Qaiser Mahmood}",
year = "2014",
month = sep,
day = "1",
doi = "10.1016/j.virep.2014.10.002",
language = "English",
volume = "3-4",
pages = "30--39",
journal = "Virology Reports",
issn = "2214-6695",
publisher = "Elsevier BV",

}

RIS

TY - JOUR

T1 - Biological and genotypic characterization of the Newcastle disease virus isolated from disease outbreaks in commercial poultry farms in northern Punjab, Pakistan

AU - Farooq, Muhammad

AU - Saliha, Uneeb

AU - Munir, Muhammad

AU - Khan, Qaiser Mahmood

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Newcastle disease (ND) is a highly contagious disease of many avian species and is particularly responsible for devastating disease outbreaks in commercial poultry flocks in Pakistan that incur huge economic losses to the national poultry industry annually. Despite implementation of an extensive vaccination program for poultry birds, the disease appears in an endemic form in commercial broiler and layer poultry farms. This study was conducted to identify the prevalent velogenic NDV strain responsible for disease outbreaks in commercial poultry farms in Punjab, Pakistan. The NDV strains isolated from pathological specimens through inoculation in embryonated chicken eggs were characterized biologically through the intracerebral pathogenicity index (ICPI), and genetically on the basis of the fusion (F) protein cleavage site. Among these, six NDV isolates showed an F protein cleavage site motif (112RRQKRF117) and an ICPI value ranging between 1.5 and 1.88, both are characteristics for velogenic strains of NDV. In addition, phylogenetic analysis based on a partial sequence of the F protein gene clustered these isolates within class II, genotype VII and specifically within genotype VII-e. This is the first report that demonstrated the presence of such NDV strains in commercial poultry farms in northern Punjab of Pakistan.

AB - Newcastle disease (ND) is a highly contagious disease of many avian species and is particularly responsible for devastating disease outbreaks in commercial poultry flocks in Pakistan that incur huge economic losses to the national poultry industry annually. Despite implementation of an extensive vaccination program for poultry birds, the disease appears in an endemic form in commercial broiler and layer poultry farms. This study was conducted to identify the prevalent velogenic NDV strain responsible for disease outbreaks in commercial poultry farms in Punjab, Pakistan. The NDV strains isolated from pathological specimens through inoculation in embryonated chicken eggs were characterized biologically through the intracerebral pathogenicity index (ICPI), and genetically on the basis of the fusion (F) protein cleavage site. Among these, six NDV isolates showed an F protein cleavage site motif (112RRQKRF117) and an ICPI value ranging between 1.5 and 1.88, both are characteristics for velogenic strains of NDV. In addition, phylogenetic analysis based on a partial sequence of the F protein gene clustered these isolates within class II, genotype VII and specifically within genotype VII-e. This is the first report that demonstrated the presence of such NDV strains in commercial poultry farms in northern Punjab of Pakistan.

KW - Newcastle disease

KW - Pakistan

KW - Pathotyping

KW - Phylogenetic analysis

KW - Poultry

KW - Sequencing

U2 - 10.1016/j.virep.2014.10.002

DO - 10.1016/j.virep.2014.10.002

M3 - Journal article

AN - SCOPUS:84919327083

VL - 3-4

SP - 30

EP - 39

JO - Virology Reports

JF - Virology Reports

SN - 2214-6695

ER -