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Calcium imaging of the cyclic nucleotide response

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter

Published
Publication date2013
Host publicationCyclic nucleotide signalling in plants
EditorsChris Gehring
Place of PublicationNew York
PublisherHumana Press
Pages107-119
Number of pages13
ISBN (Electronic)9781627034418
ISBN (Print)9781627034401
<mark>Original language</mark>English

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1016
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Abstract

Calcium (Ca2+) is s a key component of the signalling network by which plant cells respond to developmental and environmental signals. A change in guard cell cytosolic free Ca2+ ([Ca2+]cyt) is an early event in the response of stomata to both opening and closing stimuli and cyclic nucleotide-mediated Ca2+ signalling has been implicated in the regulation of stomatal aperture. A range of techniques have been used to measure [Ca2+]cyt in plant cells. Here we describe a describe a potential method for imaging cyclic nucleotide-induced changes in [Ca2+]cyt in guard cells using the cameleon ratiometric Ca2+ reporter protein.