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Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry. / Turner, K.; Porter, J.; Pickup, R. et al.
In: Journal of Applied Microbiology, Vol. 89, No. 1, 31.07.2000, p. 90-99.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Turner, K, Porter, J, Pickup, R & Edwards, C 2000, 'Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry', Journal of Applied Microbiology, vol. 89, no. 1, pp. 90-99. https://doi.org/10.1046/j.1365-2672.2000.01086.x

APA

Turner, K., Porter, J., Pickup, R., & Edwards, C. (2000). Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry. Journal of Applied Microbiology, 89(1), 90-99. https://doi.org/10.1046/j.1365-2672.2000.01086.x

Vancouver

Turner K, Porter J, Pickup R, Edwards C. Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry. Journal of Applied Microbiology. 2000 Jul 31;89(1):90-99. doi: 10.1046/j.1365-2672.2000.01086.x

Author

Turner, K. ; Porter, J. ; Pickup, R. et al. / Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry. In: Journal of Applied Microbiology. 2000 ; Vol. 89, No. 1. pp. 90-99.

Bibtex

@article{878eb9269c64427b82cb83d92f8aab8c,
title = "Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry",
abstract = "Exposure of many Gram-negative bacteria to prolonged starvation induces alternative programmes of gene expression, along with a transition into a dormant condition sometimes referred to as viable non-culturable (VBNC) state. Knowledge of how pathogenic species respond to nutrient limitation is therefore important for their detection and dissemination. This study used flow cytometry, coupled with flourescent dyes for viability and macromolecular content, to study the responses of the pathogen Salmonella typhimurium to prolonged batch culture. Statistical analysis of the flow cytometric data, together with total and culturable cell counts, failed to demonstrate a VBNC state in this pathogen, contrary to reports from other workers. Analysis of rRNA and protein content identified a small proportion of cells in 110 day-old cultures that represented an active sub-population. This observation may provide an explanation for the long-term survival properties of this organism during prolonged exposure to nutrient limitation, as well as the high degree of heterogeneity observed in labelled cells.",
author = "K. Turner and J. Porter and R. Pickup and C. Edwards",
year = "2000",
month = jul,
day = "31",
doi = "10.1046/j.1365-2672.2000.01086.x",
language = "English",
volume = "89",
pages = "90--99",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry

AU - Turner, K.

AU - Porter, J.

AU - Pickup, R.

AU - Edwards, C.

PY - 2000/7/31

Y1 - 2000/7/31

N2 - Exposure of many Gram-negative bacteria to prolonged starvation induces alternative programmes of gene expression, along with a transition into a dormant condition sometimes referred to as viable non-culturable (VBNC) state. Knowledge of how pathogenic species respond to nutrient limitation is therefore important for their detection and dissemination. This study used flow cytometry, coupled with flourescent dyes for viability and macromolecular content, to study the responses of the pathogen Salmonella typhimurium to prolonged batch culture. Statistical analysis of the flow cytometric data, together with total and culturable cell counts, failed to demonstrate a VBNC state in this pathogen, contrary to reports from other workers. Analysis of rRNA and protein content identified a small proportion of cells in 110 day-old cultures that represented an active sub-population. This observation may provide an explanation for the long-term survival properties of this organism during prolonged exposure to nutrient limitation, as well as the high degree of heterogeneity observed in labelled cells.

AB - Exposure of many Gram-negative bacteria to prolonged starvation induces alternative programmes of gene expression, along with a transition into a dormant condition sometimes referred to as viable non-culturable (VBNC) state. Knowledge of how pathogenic species respond to nutrient limitation is therefore important for their detection and dissemination. This study used flow cytometry, coupled with flourescent dyes for viability and macromolecular content, to study the responses of the pathogen Salmonella typhimurium to prolonged batch culture. Statistical analysis of the flow cytometric data, together with total and culturable cell counts, failed to demonstrate a VBNC state in this pathogen, contrary to reports from other workers. Analysis of rRNA and protein content identified a small proportion of cells in 110 day-old cultures that represented an active sub-population. This observation may provide an explanation for the long-term survival properties of this organism during prolonged exposure to nutrient limitation, as well as the high degree of heterogeneity observed in labelled cells.

U2 - 10.1046/j.1365-2672.2000.01086.x

DO - 10.1046/j.1365-2672.2000.01086.x

M3 - Journal article

C2 - 10945784

AN - SCOPUS:0033866210

VL - 89

SP - 90

EP - 99

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 1

ER -