Research output: Contribution to Journal/Magazine › Journal article
Research output: Contribution to Journal/Magazine › Journal article
}
TY - JOUR
T1 - Characterisation of a nonreducing terminal fragment from bovine articular-cartilage keratan sulfates containing alpha(2-3)-linked sialic-acid and alpha(1-3)-linked fucose.
AU - Brown, Gavin M.
AU - Huckerby, T. N.
AU - Abram, B. L.
AU - Nieduszynski, I. A.
PY - 1996/10
Y1 - 1996/10
N2 - Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6–8-year-old animals) and digested with keratanase II, an endo-b-N-acetylglucosaminidase. The resulting oligosaccharides were borohydride-reduced and fractionated on a strong anion-exchange column. 1H-NMR spectroscopic analysis of the products revealed one containing both a(2-3)-linked sialic acid and a(1-3)-linked fucose which was shown to have the structure (I) shown. This structure is a sulphated variant of the VIM-2 epitope (CD65), a putative ligand of E-selectin. No oligosaccharide containing the sialyl-Lex structure [NeuAca2-3Galb1-4(Fuca1-3)GlcNAcb1-] was identified in this study.
AB - Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6–8-year-old animals) and digested with keratanase II, an endo-b-N-acetylglucosaminidase. The resulting oligosaccharides were borohydride-reduced and fractionated on a strong anion-exchange column. 1H-NMR spectroscopic analysis of the products revealed one containing both a(2-3)-linked sialic acid and a(1-3)-linked fucose which was shown to have the structure (I) shown. This structure is a sulphated variant of the VIM-2 epitope (CD65), a putative ligand of E-selectin. No oligosaccharide containing the sialyl-Lex structure [NeuAca2-3Galb1-4(Fuca1-3)GlcNAcb1-] was identified in this study.
M3 - Journal article
VL - 319
SP - 137
EP - 141
JO - Biochemical Journal
JF - Biochemical Journal
SN - 0264-6021
IS - 1
ER -