Rights statement: This is the author’s version of a work that was accepted for publication in Environmental Pollution. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental Pollution, 220, Part B, 2017 DOI: 10.1016/j.envpol.2016.11.037
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium. / Song, Mengke; Yang, Ying; Jiang, Longfei et al.
In: Environmental Pollution, Vol. 220, No. Part B, 01.2017, p. 1059-1067.Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium
AU - Song, Mengke
AU - Yang, Ying
AU - Jiang, Longfei
AU - Hong, Qing
AU - Zhang, Dayi
AU - Shen, Zhenguo
AU - Yin, Hua
AU - Luo, Chunling
N1 - This is the author’s version of a work that was accepted for publication in Environmental Pollution. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental Pollution, 220, Part B, 2017 DOI: 10.1016/j.envpol.2016.11.037
PY - 2017/1
Y1 - 2017/1
N2 - A copper-tolerant phenanthrene (PHE)-degrading bacterium, strain Sphingobium sp. PHE-1, was newly isolated from the activated sludge in a wastewater treatment plant. Two key genes, ahdA1b-1 encoding polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHDɑ) and xyLE encoding catechol-2,3-dioxygenase (C23O), involved in the PHE metabolism by strain PHE-1 were identified. The PAH-RHD gene cluster showed 96% identity with the same cluster of Sphingomonas sp. P2. Our results indicated the induced transcription of xylE and ahdA1b-1 genes by PHE, simultaneously promoted by Cu(II). For the first time, high concentration of Cu(II) is found to encourage the expression of PAH-RHDɑ and C23O genes during PHE degradation. Applying Sphingomonas PHE-1 in PHE-contaminated soils for bioaugmentation, the abundance of xylE gene was increased by the planting of ryegrass and the presence of Cu(II), which, in turn, benefited ryegrass growth. The best performance of PHE degradation and the highest abundance of xylE genes occurred in PHE-copper co-contaminated soils planted with ryegrass.
AB - A copper-tolerant phenanthrene (PHE)-degrading bacterium, strain Sphingobium sp. PHE-1, was newly isolated from the activated sludge in a wastewater treatment plant. Two key genes, ahdA1b-1 encoding polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHDɑ) and xyLE encoding catechol-2,3-dioxygenase (C23O), involved in the PHE metabolism by strain PHE-1 were identified. The PAH-RHD gene cluster showed 96% identity with the same cluster of Sphingomonas sp. P2. Our results indicated the induced transcription of xylE and ahdA1b-1 genes by PHE, simultaneously promoted by Cu(II). For the first time, high concentration of Cu(II) is found to encourage the expression of PAH-RHDɑ and C23O genes during PHE degradation. Applying Sphingomonas PHE-1 in PHE-contaminated soils for bioaugmentation, the abundance of xylE gene was increased by the planting of ryegrass and the presence of Cu(II), which, in turn, benefited ryegrass growth. The best performance of PHE degradation and the highest abundance of xylE genes occurred in PHE-copper co-contaminated soils planted with ryegrass.
KW - Bioremediation
KW - Phenanthrene (PHE)
KW - Copper
KW - Sphingobium
KW - PAH-RHDα gene
KW - C23O gene
U2 - 10.1016/j.envpol.2016.11.037
DO - 10.1016/j.envpol.2016.11.037
M3 - Journal article
VL - 220
SP - 1059
EP - 1067
JO - Environmental Pollution
JF - Environmental Pollution
SN - 0269-7491
IS - Part B
ER -