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    Rights statement: This is the author’s version of a work that was accepted for publication in Environmental Pollution. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental Pollution, 220, Part B, 2017 DOI: 10.1016/j.envpol.2016.11.037

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Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium. / Song, Mengke; Yang, Ying; Jiang, Longfei et al.
In: Environmental Pollution, Vol. 220, No. Part B, 01.2017, p. 1059-1067.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Song, M, Yang, Y, Jiang, L, Hong, Q, Zhang, D, Shen, Z, Yin, H & Luo, C 2017, 'Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium', Environmental Pollution, vol. 220, no. Part B, pp. 1059-1067. https://doi.org/10.1016/j.envpol.2016.11.037

APA

Song, M., Yang, Y., Jiang, L., Hong, Q., Zhang, D., Shen, Z., Yin, H., & Luo, C. (2017). Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium. Environmental Pollution, 220(Part B), 1059-1067. https://doi.org/10.1016/j.envpol.2016.11.037

Vancouver

Song M, Yang Y, Jiang L, Hong Q, Zhang D, Shen Z et al. Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium. Environmental Pollution. 2017 Jan;220(Part B):1059-1067. Epub 2016 Nov 23. doi: 10.1016/j.envpol.2016.11.037

Author

Song, Mengke ; Yang, Ying ; Jiang, Longfei et al. / Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium. In: Environmental Pollution. 2017 ; Vol. 220, No. Part B. pp. 1059-1067.

Bibtex

@article{5a9ff4ff9ecd4d44944ab80f4a2417d3,
title = "Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium",
abstract = "A copper-tolerant phenanthrene (PHE)-degrading bacterium, strain Sphingobium sp. PHE-1, was newly isolated from the activated sludge in a wastewater treatment plant. Two key genes, ahdA1b-1 encoding polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHDɑ) and xyLE encoding catechol-2,3-dioxygenase (C23O), involved in the PHE metabolism by strain PHE-1 were identified. The PAH-RHD gene cluster showed 96% identity with the same cluster of Sphingomonas sp. P2. Our results indicated the induced transcription of xylE and ahdA1b-1 genes by PHE, simultaneously promoted by Cu(II). For the first time, high concentration of Cu(II) is found to encourage the expression of PAH-RHDɑ and C23O genes during PHE degradation. Applying Sphingomonas PHE-1 in PHE-contaminated soils for bioaugmentation, the abundance of xylE gene was increased by the planting of ryegrass and the presence of Cu(II), which, in turn, benefited ryegrass growth. The best performance of PHE degradation and the highest abundance of xylE genes occurred in PHE-copper co-contaminated soils planted with ryegrass.",
keywords = "Bioremediation, Phenanthrene (PHE), Copper, Sphingobium, PAH-RHDα gene, C23O gene",
author = "Mengke Song and Ying Yang and Longfei Jiang and Qing Hong and Dayi Zhang and Zhenguo Shen and Hua Yin and Chunling Luo",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Environmental Pollution. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental Pollution, 220, Part B, 2017 DOI: 10.1016/j.envpol.2016.11.037",
year = "2017",
month = jan,
doi = "10.1016/j.envpol.2016.11.037",
language = "English",
volume = "220",
pages = "1059--1067",
journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "Elsevier Ltd",
number = "Part B",

}

RIS

TY - JOUR

T1 - Characterisation of the phenanthrene degradation-related genes and degrading ability of a newly isolated copper-tolerant bacterium

AU - Song, Mengke

AU - Yang, Ying

AU - Jiang, Longfei

AU - Hong, Qing

AU - Zhang, Dayi

AU - Shen, Zhenguo

AU - Yin, Hua

AU - Luo, Chunling

N1 - This is the author’s version of a work that was accepted for publication in Environmental Pollution. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental Pollution, 220, Part B, 2017 DOI: 10.1016/j.envpol.2016.11.037

PY - 2017/1

Y1 - 2017/1

N2 - A copper-tolerant phenanthrene (PHE)-degrading bacterium, strain Sphingobium sp. PHE-1, was newly isolated from the activated sludge in a wastewater treatment plant. Two key genes, ahdA1b-1 encoding polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHDɑ) and xyLE encoding catechol-2,3-dioxygenase (C23O), involved in the PHE metabolism by strain PHE-1 were identified. The PAH-RHD gene cluster showed 96% identity with the same cluster of Sphingomonas sp. P2. Our results indicated the induced transcription of xylE and ahdA1b-1 genes by PHE, simultaneously promoted by Cu(II). For the first time, high concentration of Cu(II) is found to encourage the expression of PAH-RHDɑ and C23O genes during PHE degradation. Applying Sphingomonas PHE-1 in PHE-contaminated soils for bioaugmentation, the abundance of xylE gene was increased by the planting of ryegrass and the presence of Cu(II), which, in turn, benefited ryegrass growth. The best performance of PHE degradation and the highest abundance of xylE genes occurred in PHE-copper co-contaminated soils planted with ryegrass.

AB - A copper-tolerant phenanthrene (PHE)-degrading bacterium, strain Sphingobium sp. PHE-1, was newly isolated from the activated sludge in a wastewater treatment plant. Two key genes, ahdA1b-1 encoding polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase (PAH-RHDɑ) and xyLE encoding catechol-2,3-dioxygenase (C23O), involved in the PHE metabolism by strain PHE-1 were identified. The PAH-RHD gene cluster showed 96% identity with the same cluster of Sphingomonas sp. P2. Our results indicated the induced transcription of xylE and ahdA1b-1 genes by PHE, simultaneously promoted by Cu(II). For the first time, high concentration of Cu(II) is found to encourage the expression of PAH-RHDɑ and C23O genes during PHE degradation. Applying Sphingomonas PHE-1 in PHE-contaminated soils for bioaugmentation, the abundance of xylE gene was increased by the planting of ryegrass and the presence of Cu(II), which, in turn, benefited ryegrass growth. The best performance of PHE degradation and the highest abundance of xylE genes occurred in PHE-copper co-contaminated soils planted with ryegrass.

KW - Bioremediation

KW - Phenanthrene (PHE)

KW - Copper

KW - Sphingobium

KW - PAH-RHDα gene

KW - C23O gene

U2 - 10.1016/j.envpol.2016.11.037

DO - 10.1016/j.envpol.2016.11.037

M3 - Journal article

VL - 220

SP - 1059

EP - 1067

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

IS - Part B

ER -