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Characterization and expression of an mRNA encoding a wound-induced (win) protein from ethylene-treated tomato leaf abscission zone tissue

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<mark>Journal publication date</mark>1/06/1997
<mark>Journal</mark>Journal of Experimental Botany
Issue number6
Volume48
Number of pages5
Pages (from-to)1223-1227
Publication StatusPublished
<mark>Original language</mark>English

Abstract

A cDNA clone (TAB7) encoding a putative wound-induced (Win) protein has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) leaf abscission zone cDNA library using a differential screening strategy. The clone has a high degree of homology at the amino acid level to both the potato win1 and 2 genes, Hevea brasiliensis hevein and Nicotiana tabacum PR-4a and PR-4b proteins. The mRNA encoded by TAB7 is up-regulated within 12 h of exposure to ethylene (10 μl l-1) and its expression increases steadily within the cells comprising the leaf abscission zone and to a lesser extent in the adjacent non-zone tissue. This rise precedes the onset of cell separation. Southern analysis indicates that the mRNA is encoded by either a single gene or a small gene family. The role of the protein during abscission is discussed.