Characterization and expression of an mRNA encoding a wound-induced (win) protein from ethylene-treated tomato leaf abscission zone tissue

Lancaster Environment Centre

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https://doi.org/10.1093/jxb/48.6.1223
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Keywords

Abscission zone, Ethylene, Lycopersicon esculentum, Tomato, Wound-induced proteins

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Characterization and expression of an mRNA encoding a wound-induced (win) protein from ethylene-treated tomato leaf abscission zone tissue. / Harris, Neil; Taylor, Jane E.; Roberts, Jeremy A.
In: Journal of Experimental Botany, Vol. 48, No. 6, 01.06.1997, p. 1223-1227.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{d0746a6d577d4fb39989c91bdd0b9a5a,

title = "Characterization and expression of an mRNA encoding a wound-induced (win) protein from ethylene-treated tomato leaf abscission zone tissue",

abstract = "A cDNA clone (TAB7) encoding a putative wound-induced (Win) protein has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) leaf abscission zone cDNA library using a differential screening strategy. The clone has a high degree of homology at the amino acid level to both the potato win1 and 2 genes, Hevea brasiliensis hevein and Nicotiana tabacum PR-4a and PR-4b proteins. The mRNA encoded by TAB7 is up-regulated within 12 h of exposure to ethylene (10 μl l-1) and its expression increases steadily within the cells comprising the leaf abscission zone and to a lesser extent in the adjacent non-zone tissue. This rise precedes the onset of cell separation. Southern analysis indicates that the mRNA is encoded by either a single gene or a small gene family. The role of the protein during abscission is discussed.",

keywords = "Abscission zone, Ethylene, Lycopersicon esculentum, Tomato, Wound-induced proteins",

author = "Neil Harris and Taylor, {Jane E.} and Roberts, {Jeremy A.}",

year = "1997",

month = jun,

day = "1",

doi = "10.1093/jxb/48.6.1223",

language = "English",

volume = "48",

pages = "1223--1227",

journal = "Journal of Experimental Botany",

issn = "0022-0957",

publisher = "OXFORD UNIV PRESS",

number = "6",

}

RIS

TY - JOUR

T1 - Characterization and expression of an mRNA encoding a wound-induced (win) protein from ethylene-treated tomato leaf abscission zone tissue

AU - Harris, Neil

AU - Taylor, Jane E.

AU - Roberts, Jeremy A.

PY - 1997/6/1

Y1 - 1997/6/1

N2 - A cDNA clone (TAB7) encoding a putative wound-induced (Win) protein has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) leaf abscission zone cDNA library using a differential screening strategy. The clone has a high degree of homology at the amino acid level to both the potato win1 and 2 genes, Hevea brasiliensis hevein and Nicotiana tabacum PR-4a and PR-4b proteins. The mRNA encoded by TAB7 is up-regulated within 12 h of exposure to ethylene (10 μl l-1) and its expression increases steadily within the cells comprising the leaf abscission zone and to a lesser extent in the adjacent non-zone tissue. This rise precedes the onset of cell separation. Southern analysis indicates that the mRNA is encoded by either a single gene or a small gene family. The role of the protein during abscission is discussed.

AB - A cDNA clone (TAB7) encoding a putative wound-induced (Win) protein has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) leaf abscission zone cDNA library using a differential screening strategy. The clone has a high degree of homology at the amino acid level to both the potato win1 and 2 genes, Hevea brasiliensis hevein and Nicotiana tabacum PR-4a and PR-4b proteins. The mRNA encoded by TAB7 is up-regulated within 12 h of exposure to ethylene (10 μl l-1) and its expression increases steadily within the cells comprising the leaf abscission zone and to a lesser extent in the adjacent non-zone tissue. This rise precedes the onset of cell separation. Southern analysis indicates that the mRNA is encoded by either a single gene or a small gene family. The role of the protein during abscission is discussed.

KW - Abscission zone

KW - Ethylene

KW - Lycopersicon esculentum

KW - Tomato

KW - Wound-induced proteins

U2 - 10.1093/jxb/48.6.1223

DO - 10.1093/jxb/48.6.1223

M3 - Journal article

AN - SCOPUS:0030738262

VL - 48

SP - 1223

EP - 1227

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 6

ER -

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