Home > Research > Publications & Outputs > Characterization of a novel member of genus Ifl...

Electronic data

  • 1-s2.0-S0022201117300241-main

    Rights statement: This is the author’s version of a work that was accepted for publication in Journal of Invertebrate Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Invertebrate Pathology, 144, 2017 DOI: 10.1016/j.jip.2017.01.011

    Accepted author manuscript, 2.17 MB, PDF document

    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Links

Text available via DOI:

View graph of relations

Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

Research output: Contribution to journalJournal articlepeer-review

Published
Close
<mark>Journal publication date</mark>03/2017
<mark>Journal</mark>Journal of Invertebrate Pathology
Volume144
Number of pages9
Pages (from-to)65-73
Publication StatusPublished
Early online date3/02/17
<mark>Original language</mark>English

Abstract

The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3,021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 108 copies /μl) while vertical transmission efficiency was found to be relatively low (< 28.57%). These results suggest that we have found a novel member of genus Iflavirus in H. armigera.

Bibliographic note

This is the author’s version of a work that was accepted for publication in Journal of Invertebrate Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Invertebrate Pathology, 144, 2017 DOI: 10.1016/j.jip.2017.01.011