Home > Research > Publications & Outputs > Characterization of a novel member of genus Ifl...

Electronic data

  • 1-s2.0-S0022201117300241-main

    Rights statement: This is the author’s version of a work that was accepted for publication in Journal of Invertebrate Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Invertebrate Pathology, 144, 2017 DOI: 10.1016/j.jip.2017.01.011

    Accepted author manuscript, 2.17 MB, PDF document

    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Links

Text available via DOI:

View graph of relations

Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Characterization of a novel member of genus Iflavirus in Helicoverpa armigera. / Yuan, He; Xu, Pengjun; [Unknown], XianmingYang et al.

In: Journal of Invertebrate Pathology, Vol. 144, 03.2017, p. 65-73.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Yuan, H, Xu, P, [Unknown], X, Graham, RI, Wilson, K & Wu, K 2017, 'Characterization of a novel member of genus Iflavirus in Helicoverpa armigera', Journal of Invertebrate Pathology, vol. 144, pp. 65-73. https://doi.org/10.1016/j.jip.2017.01.011

APA

Yuan, H., Xu, P., [Unknown], X., Graham, R. I., Wilson, K., & Wu, K. (2017). Characterization of a novel member of genus Iflavirus in Helicoverpa armigera. Journal of Invertebrate Pathology, 144, 65-73. https://doi.org/10.1016/j.jip.2017.01.011

Vancouver

Yuan H, Xu P, [Unknown] X, Graham RI, Wilson K, Wu K. Characterization of a novel member of genus Iflavirus in Helicoverpa armigera. Journal of Invertebrate Pathology. 2017 Mar;144:65-73. Epub 2017 Feb 3. doi: 10.1016/j.jip.2017.01.011

Author

Yuan, He ; Xu, Pengjun ; [Unknown], XianmingYang et al. / Characterization of a novel member of genus Iflavirus in Helicoverpa armigera. In: Journal of Invertebrate Pathology. 2017 ; Vol. 144. pp. 65-73.

Bibtex

@article{f8be80a9da634d988ddea7e8e45f6d9c,
title = "Characterization of a novel member of genus Iflavirus in Helicoverpa armigera",
abstract = "The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3,021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 108 copies /μl) while vertical transmission efficiency was found to be relatively low (< 28.57%). These results suggest that we have found a novel member of genus Iflavirus in H. armigera.",
keywords = "Helicoverpa armigera, iflavirus, RNA virus, horizontal transmission, vertical transmission, tissue distribution",
author = "He Yuan and Pengjun Xu and XianmingYang [Unknown] and Graham, {Robert I.} and Kenneth Wilson and Kongming Wu",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Journal of Invertebrate Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Invertebrate Pathology, 144, 2017 DOI: 10.1016/j.jip.2017.01.011",
year = "2017",
month = mar,
doi = "10.1016/j.jip.2017.01.011",
language = "English",
volume = "144",
pages = "65--73",
journal = "Journal of Invertebrate Pathology",
issn = "0022-2011",
publisher = "ELSEVIER ACADEMIC PRESS INC",

}

RIS

TY - JOUR

T1 - Characterization of a novel member of genus Iflavirus in Helicoverpa armigera

AU - Yuan, He

AU - Xu, Pengjun

AU - [Unknown], XianmingYang

AU - Graham, Robert I.

AU - Wilson, Kenneth

AU - Wu, Kongming

N1 - This is the author’s version of a work that was accepted for publication in Journal of Invertebrate Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Invertebrate Pathology, 144, 2017 DOI: 10.1016/j.jip.2017.01.011

PY - 2017/3

Y1 - 2017/3

N2 - The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3,021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 108 copies /μl) while vertical transmission efficiency was found to be relatively low (< 28.57%). These results suggest that we have found a novel member of genus Iflavirus in H. armigera.

AB - The cotton bollworm, Helicoverpa armigera, is one of the most important agricultural pests of many economic crops worldwide. Herein, we found a novel single-strand RNA virus by RNA-Seq and Polymerase Chain Reaction (PCR) method in H. armigera named Helicoverpa armigera iflavirus (HaIV), which possessed a genome with 10,017 nucleotides in length and contained a single large open reading frame (ORF) encoding a putative polyprotein of 3,021 amino acids with a predicted molecular mass of 344.16 kDa and a theoretical isoelectric point (pI) of 6.45. The deduced amino acid sequence showed highest similarity (61.0%) with the protein of Lymantria dispar Iflavirus 1. Phylogenetic analysis with putative RdRp amino acid sequences indicated that the virus clustered with members of the genus Iflavirus. The virus was mainly distributed in the fat body of its host and was found to be capable of both horizontal and vertical transmission. The efficiency of perorally horizontal transmission was dose dependent (100% infection rate with a viral dose of 108 copies /μl) while vertical transmission efficiency was found to be relatively low (< 28.57%). These results suggest that we have found a novel member of genus Iflavirus in H. armigera.

KW - Helicoverpa armigera

KW - iflavirus

KW - RNA virus

KW - horizontal transmission

KW - vertical transmission

KW - tissue distribution

U2 - 10.1016/j.jip.2017.01.011

DO - 10.1016/j.jip.2017.01.011

M3 - Journal article

VL - 144

SP - 65

EP - 73

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

SN - 0022-2011

ER -