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Chitosan/hydroxyapatite (HA)/hydroxypropylmethyl cellulose (HPMC) spongy scaffolds-synthesis and evaluation as potential alveolar bone substitutes

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Chitosan/hydroxyapatite (HA)/hydroxypropylmethyl cellulose (HPMC) spongy scaffolds-synthesis and evaluation as potential alveolar bone substitutes. / Iqbal, Haffsah; Ali, Moazzam; Zeeshan, Rabia; Mutahir, Zeeshan; Iqbal, Farasat; Nawaz, Muhammad Azhar Hayat; Shahzadi, Lubna; Chaudhry, Aqif Anwar; Yar, Muhammad; Luan, Shifang; Khan, Ather Farooq; Rehman, Ihtesham-Ur.

In: Colloids and Surfaces B: Biointerfaces, Vol. 160, 01.12.2017, p. 553-563.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Iqbal, H, Ali, M, Zeeshan, R, Mutahir, Z, Iqbal, F, Nawaz, MAH, Shahzadi, L, Chaudhry, AA, Yar, M, Luan, S, Khan, AF & Rehman, I-U 2017, 'Chitosan/hydroxyapatite (HA)/hydroxypropylmethyl cellulose (HPMC) spongy scaffolds-synthesis and evaluation as potential alveolar bone substitutes', Colloids and Surfaces B: Biointerfaces, vol. 160, pp. 553-563. https://doi.org/10.1016/j.colsurfb.2017.09.059

APA

Iqbal, H., Ali, M., Zeeshan, R., Mutahir, Z., Iqbal, F., Nawaz, M. A. H., Shahzadi, L., Chaudhry, A. A., Yar, M., Luan, S., Khan, A. F., & Rehman, I-U. (2017). Chitosan/hydroxyapatite (HA)/hydroxypropylmethyl cellulose (HPMC) spongy scaffolds-synthesis and evaluation as potential alveolar bone substitutes. Colloids and Surfaces B: Biointerfaces, 160, 553-563. https://doi.org/10.1016/j.colsurfb.2017.09.059

Vancouver

Iqbal H, Ali M, Zeeshan R, Mutahir Z, Iqbal F, Nawaz MAH et al. Chitosan/hydroxyapatite (HA)/hydroxypropylmethyl cellulose (HPMC) spongy scaffolds-synthesis and evaluation as potential alveolar bone substitutes. Colloids and Surfaces B: Biointerfaces. 2017 Dec 1;160:553-563. https://doi.org/10.1016/j.colsurfb.2017.09.059

Author

Iqbal, Haffsah ; Ali, Moazzam ; Zeeshan, Rabia ; Mutahir, Zeeshan ; Iqbal, Farasat ; Nawaz, Muhammad Azhar Hayat ; Shahzadi, Lubna ; Chaudhry, Aqif Anwar ; Yar, Muhammad ; Luan, Shifang ; Khan, Ather Farooq ; Rehman, Ihtesham-Ur. / Chitosan/hydroxyapatite (HA)/hydroxypropylmethyl cellulose (HPMC) spongy scaffolds-synthesis and evaluation as potential alveolar bone substitutes. In: Colloids and Surfaces B: Biointerfaces. 2017 ; Vol. 160. pp. 553-563.

Bibtex

@article{9c3681442ad74bd5968d9bf599a9c844,
title = "Chitosan/hydroxyapatite (HA)/hydroxypropylmethyl cellulose (HPMC) spongy scaffolds-synthesis and evaluation as potential alveolar bone substitutes",
abstract = "Alveolar bone loss is associated with infections and its augmentation is a pre-requisite for the success of dental implants. In present study, we aim to develop and evaluate novel freeze dried doxycycline loaded chitosan (CS)/hydroxyapatite (HA) spongy scaffolds where hydroxypropylmethyl cellulose (HPMC) was added as a crosslinker. Scaffolds displayed compressive strength of 14MPa/cm3 and 0.34 as elastic response. The interconnected pore diameter was 41-273μm, favorably provided the template supporting cells and transport. An overall 10% degradation was seen after 14day's studies at pH 7.4 in PBS. Doxycycline hyclate, a frequently used drug to counter oral infections, demonstrated an initial burst release (6-8h), followed by a sustain release profile for the remaining 64h. CS/HA/HPMC scaffolds were nontoxic and promoted pre-osteoblast cell viability as seen with live/dead calcein staining after 24h where scaffolds with 10% and 25% HPMC by weight of scaffold had more viable cells. Scaffolds with 10%, 20% and 25% HPMC by weight of scaffold showed efficient cellular adhesion as seen in scanning electron microscopy images (day 8) indicating that pre-osteoblast cells were able to adhere well on the surface and into the porous structure via cytoplasmic extensions. Hoechst 33258 nuclear staining at day 2 and 8 indicated cell proliferation which was further supported byMTT assay at day 2, 4 and 8. Although all scaffolds supported pre-osteoblast cell viability, alkaline phosphatase (ALP) staining demonstrated that upon induction, differentiation was pronounced in case of scaffolds with 10% HMPC scaffolds. Conclusively, these materials having all the required mechanical and biological properties are potential candidates for alveolar bone regeneration.",
keywords = "Algorithms, Animals, Anti-Bacterial Agents/chemistry, Biocompatible Materials/chemistry, Bone Substitutes/chemistry, Cell Adhesion/drug effects, Cell Line, Cell Proliferation/drug effects, Chitosan/chemistry, Doxycycline/chemistry, Drug Liberation, Durapatite/chemistry, Freeze Drying, Hypromellose Derivatives/chemistry, Mice, Osteoblasts/cytology, Tissue Engineering/methods, Tissue Scaffolds/chemistry",
author = "Haffsah Iqbal and Moazzam Ali and Rabia Zeeshan and Zeeshan Mutahir and Farasat Iqbal and Nawaz, {Muhammad Azhar Hayat} and Lubna Shahzadi and Chaudhry, {Aqif Anwar} and Muhammad Yar and Shifang Luan and Khan, {Ather Farooq} and Ihtesham-Ur Rehman",
year = "2017",
month = dec,
day = "1",
doi = "10.1016/j.colsurfb.2017.09.059",
language = "English",
volume = "160",
pages = "553--563",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier Science B.V.",

}

RIS

TY - JOUR

T1 - Chitosan/hydroxyapatite (HA)/hydroxypropylmethyl cellulose (HPMC) spongy scaffolds-synthesis and evaluation as potential alveolar bone substitutes

AU - Iqbal, Haffsah

AU - Ali, Moazzam

AU - Zeeshan, Rabia

AU - Mutahir, Zeeshan

AU - Iqbal, Farasat

AU - Nawaz, Muhammad Azhar Hayat

AU - Shahzadi, Lubna

AU - Chaudhry, Aqif Anwar

AU - Yar, Muhammad

AU - Luan, Shifang

AU - Khan, Ather Farooq

AU - Rehman, Ihtesham-Ur

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Alveolar bone loss is associated with infections and its augmentation is a pre-requisite for the success of dental implants. In present study, we aim to develop and evaluate novel freeze dried doxycycline loaded chitosan (CS)/hydroxyapatite (HA) spongy scaffolds where hydroxypropylmethyl cellulose (HPMC) was added as a crosslinker. Scaffolds displayed compressive strength of 14MPa/cm3 and 0.34 as elastic response. The interconnected pore diameter was 41-273μm, favorably provided the template supporting cells and transport. An overall 10% degradation was seen after 14day's studies at pH 7.4 in PBS. Doxycycline hyclate, a frequently used drug to counter oral infections, demonstrated an initial burst release (6-8h), followed by a sustain release profile for the remaining 64h. CS/HA/HPMC scaffolds were nontoxic and promoted pre-osteoblast cell viability as seen with live/dead calcein staining after 24h where scaffolds with 10% and 25% HPMC by weight of scaffold had more viable cells. Scaffolds with 10%, 20% and 25% HPMC by weight of scaffold showed efficient cellular adhesion as seen in scanning electron microscopy images (day 8) indicating that pre-osteoblast cells were able to adhere well on the surface and into the porous structure via cytoplasmic extensions. Hoechst 33258 nuclear staining at day 2 and 8 indicated cell proliferation which was further supported byMTT assay at day 2, 4 and 8. Although all scaffolds supported pre-osteoblast cell viability, alkaline phosphatase (ALP) staining demonstrated that upon induction, differentiation was pronounced in case of scaffolds with 10% HMPC scaffolds. Conclusively, these materials having all the required mechanical and biological properties are potential candidates for alveolar bone regeneration.

AB - Alveolar bone loss is associated with infections and its augmentation is a pre-requisite for the success of dental implants. In present study, we aim to develop and evaluate novel freeze dried doxycycline loaded chitosan (CS)/hydroxyapatite (HA) spongy scaffolds where hydroxypropylmethyl cellulose (HPMC) was added as a crosslinker. Scaffolds displayed compressive strength of 14MPa/cm3 and 0.34 as elastic response. The interconnected pore diameter was 41-273μm, favorably provided the template supporting cells and transport. An overall 10% degradation was seen after 14day's studies at pH 7.4 in PBS. Doxycycline hyclate, a frequently used drug to counter oral infections, demonstrated an initial burst release (6-8h), followed by a sustain release profile for the remaining 64h. CS/HA/HPMC scaffolds were nontoxic and promoted pre-osteoblast cell viability as seen with live/dead calcein staining after 24h where scaffolds with 10% and 25% HPMC by weight of scaffold had more viable cells. Scaffolds with 10%, 20% and 25% HPMC by weight of scaffold showed efficient cellular adhesion as seen in scanning electron microscopy images (day 8) indicating that pre-osteoblast cells were able to adhere well on the surface and into the porous structure via cytoplasmic extensions. Hoechst 33258 nuclear staining at day 2 and 8 indicated cell proliferation which was further supported byMTT assay at day 2, 4 and 8. Although all scaffolds supported pre-osteoblast cell viability, alkaline phosphatase (ALP) staining demonstrated that upon induction, differentiation was pronounced in case of scaffolds with 10% HMPC scaffolds. Conclusively, these materials having all the required mechanical and biological properties are potential candidates for alveolar bone regeneration.

KW - Algorithms

KW - Animals

KW - Anti-Bacterial Agents/chemistry

KW - Biocompatible Materials/chemistry

KW - Bone Substitutes/chemistry

KW - Cell Adhesion/drug effects

KW - Cell Line

KW - Cell Proliferation/drug effects

KW - Chitosan/chemistry

KW - Doxycycline/chemistry

KW - Drug Liberation

KW - Durapatite/chemistry

KW - Freeze Drying

KW - Hypromellose Derivatives/chemistry

KW - Mice

KW - Osteoblasts/cytology

KW - Tissue Engineering/methods

KW - Tissue Scaffolds/chemistry

U2 - 10.1016/j.colsurfb.2017.09.059

DO - 10.1016/j.colsurfb.2017.09.059

M3 - Journal article

C2 - 29024920

VL - 160

SP - 553

EP - 563

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

SN - 0927-7765

ER -