Training for both sporting and military performance is common practice within army trainee populations, although it is currently unknown what effect this combination of training methods may have on the physical attributes required for overall physical preparedness. This study examined the effects of sport-specific training on general fitness in a professional military population. Four hundred and twenty-three Greek male army cadets completed a 12-week training regimen involving standard physical training (callisthenics, strength and endurance running exercises) and either general military training (GMT) or sport military training (SMT). A series of physical tests took place before and after the training period: a mile run, pull-ups, 50 m swim and an obstacle course run. Both the GMT and SMT groups showed significant (p < 0.001) improvements in all physical tests. However, the SMT group produced significantly greater improvements in all four tests (pull-ups [p < 0.001], 50 m swim [p < 0.05], obstacle course [p < 0.01] and mile run [p < 0.01]) compared to the GMT group. Furthermore, different types of SMT (e.g. rock climbing and track sprinting) achieved greater improvements (p < 0.001–0.01) in certain physical tests when compared to other forms of SMT (e.g. Pankration, Fencing). These results indicate that cadets undertaking concurrent participation in general and sport military training are overall better prepared for physical performance than their counterparts who undertake only general military training. Military conditioning personnel should be aware of the positive interplay between general and sports specific training in forming a preparation strategy designed for physical performance.