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CONFOCAL AND CONVENTIONAL IMMUNOFLUORESCENT AND IMMUNOGOLD ELECTRON-MICROSCOPIC LOCALIZATION OF COLLAGEN TYPE-III AND TYPE-IV IN HUMAN PLACENTA

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  • A K NANAEV
  • V S RUKOSUEV
  • V P SHIRINSKY
  • A P MILOVANOV
  • S P DOMOGATSKY
  • V C DUANCE
  • F M BRADBURY
  • P YARROW
  • L GARDINER
  • C DLACEY
  • C D OCKLEFORD
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<mark>Journal publication date</mark>11/1991
<mark>Journal</mark>Placenta
Issue number6
Volume12
Number of pages23
Pages (from-to)573-595
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Confocal and conventional indirect immunofluorescence and immunogold electron microscopic methods were applied to examine the distribution of extracellular matrix constituents (collagens types III and ITq in the villi of immature and term human placentae. The immunofluorescence study revealed that collagen type III is more distinct in the villous stroma of terns placenta as compared with that of the first trimester. Collagen type IV was detected mainly in endothelial and epithelial basement membranes and interestingly also to a certain extent in the stroma. Results obtained using immunoelectron microscopy support the proposal that collagen types III and IV are characteristic of stromal and basement membranes, respectively. Stromal collagen type IV is apparently localized in association with the interstitial types of collagen (I and III), in the villous stroma of term placenta.