Research output: Contribution to Journal/Magazine › Journal article › peer-review
CONFOCAL AND CONVENTIONAL IMMUNOFLUORESCENT AND IMMUNOGOLD ELECTRON-MICROSCOPIC LOCALIZATION OF COLLAGEN TYPE-III AND TYPE-IV IN HUMAN PLACENTA. / NANAEV, A K ; RUKOSUEV, V S ; SHIRINSKY, V P et al.
In: Placenta, Vol. 12, No. 6, 11.1991, p. 573-595.Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - CONFOCAL AND CONVENTIONAL IMMUNOFLUORESCENT AND IMMUNOGOLD ELECTRON-MICROSCOPIC LOCALIZATION OF COLLAGEN TYPE-III AND TYPE-IV IN HUMAN PLACENTA
AU - NANAEV, A K
AU - RUKOSUEV, V S
AU - SHIRINSKY, V P
AU - MILOVANOV, A P
AU - DOMOGATSKY, S P
AU - DUANCE, V C
AU - BRADBURY, F M
AU - YARROW, P
AU - GARDINER, L
AU - DLACEY, C
AU - OCKLEFORD, C D
PY - 1991/11
Y1 - 1991/11
N2 - Confocal and conventional indirect immunofluorescence and immunogold electron microscopic methods were applied to examine the distribution of extracellular matrix constituents (collagens types III and ITq in the villi of immature and term human placentae. The immunofluorescence study revealed that collagen type III is more distinct in the villous stroma of terns placenta as compared with that of the first trimester. Collagen type IV was detected mainly in endothelial and epithelial basement membranes and interestingly also to a certain extent in the stroma. Results obtained using immunoelectron microscopy support the proposal that collagen types III and IV are characteristic of stromal and basement membranes, respectively. Stromal collagen type IV is apparently localized in association with the interstitial types of collagen (I and III), in the villous stroma of term placenta.
AB - Confocal and conventional indirect immunofluorescence and immunogold electron microscopic methods were applied to examine the distribution of extracellular matrix constituents (collagens types III and ITq in the villi of immature and term human placentae. The immunofluorescence study revealed that collagen type III is more distinct in the villous stroma of terns placenta as compared with that of the first trimester. Collagen type IV was detected mainly in endothelial and epithelial basement membranes and interestingly also to a certain extent in the stroma. Results obtained using immunoelectron microscopy support the proposal that collagen types III and IV are characteristic of stromal and basement membranes, respectively. Stromal collagen type IV is apparently localized in association with the interstitial types of collagen (I and III), in the villous stroma of term placenta.
KW - BASEMENT-MEMBRANE COMPONENTS
KW - EXTRACELLULAR-MATRIX
KW - CHORIONIC VILLI
KW - MESSENGER-RNA
KW - LYMPH-NODE
KW - PROTEINS
KW - LAMININ
KW - IDENTIFICATION
KW - ULTRASTRUCTURE
KW - FIBRONECTIN
U2 - 10.1016/0143-4004(91)90493-Y
DO - 10.1016/0143-4004(91)90493-Y
M3 - Journal article
VL - 12
SP - 573
EP - 595
JO - Placenta
JF - Placenta
SN - 0143-4004
IS - 6
ER -