TY - JOUR

T1 - Confocal and fluorescence lifetime imaging sheds light on the fate of a pyrene-tagged carbon monoxide-releasing Fischer carbene chromium complex

AU - Arrowsmith, Rory L.

AU - Atkin, Anthony J.

AU - Botchway, Stanley W.

AU - Fairlamb, Ian J. S.

AU - Lynam, Jason M.

AU - Moir, James W. B.

AU - Pascu, Sofia I.

AU - Ward, Jonathan S.

AU - Zhang, Wei-Qiang

PY - 2015/3/21

Y1 - 2015/3/21

N2 - The synthesis of a new pyrene-containing Fischer carbene complex is described. The complex has a broad absorbance spectrum between 300 and 400 nm and, on excitation at 345 nm in CH2Cl2 solution, emission is observed at 395 and 415 nm. Emission is also observed in PBS buffer, but in this case the resulting spectra are much broader. Confocal and fluorescence lifetime imaging indicate that emission occurs on treating HeLa cells with the complex and co-localisation studies demonstrate that this is from the mitochondria and lipid-rich regions of the cell.

AB - The synthesis of a new pyrene-containing Fischer carbene complex is described. The complex has a broad absorbance spectrum between 300 and 400 nm and, on excitation at 345 nm in CH2Cl2 solution, emission is observed at 395 and 415 nm. Emission is also observed in PBS buffer, but in this case the resulting spectra are much broader. Confocal and fluorescence lifetime imaging indicate that emission occurs on treating HeLa cells with the complex and co-localisation studies demonstrate that this is from the mitochondria and lipid-rich regions of the cell.

U2 - 10.1039/c4dt03312d

DO - 10.1039/c4dt03312d

M3 - Journal article

VL - 44

SP - 4957

EP - 4962

JO - Dalton Transactions

JF - Dalton Transactions

SN - 1477-9226

IS - 11

ER -