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Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter (peer-reviewed)peer-review

Published
Publication date30/07/2018
Host publicationPhotosynthesis
EditorsS. Covshoff
Place of PublicationNew York
PublisherHumana Press
Pages253-261
Number of pages9
ISBN (Electronic)9781493977864
ISBN (Print)9781493977857
<mark>Original language</mark>English

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1770
ISSN (Print)1064-3745

Abstract

Imaging of mesophyll cell suspensions prepared from Arabidopsis has been pivotal for forming our current understanding of the molecular control of chloroplast division over the past 25 years. In this chapter, we provide a method for the preparation of leaf cell suspensions that improves upon a previous method by optimizing cellular preservation and cell separation. This technique is accessible to all researchers and amenable for use with all plant species. The leaf suspensions can be used for imaging chloroplast features within a cell that are important for photosynthesis such as size, number, and distribution. However, we also provide examples to illustrate how the cells in the suspensions can be easily stained to image other features, for example pit fields where plasmodesmata are located and organelles such as mitochondria, to improve our understanding of traits that are important for photosynthetic physiology.