Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

Published

Standard

Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features. / Khoshravesh, Roxana; Sage, Tammy L.
Photosynthesis. ed. / S. Covshoff. New York: Humana Press, 2018. p. 253-261 (Methods in Molecular Biology; Vol. 1770).

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter (peer-reviewed) › peer-review

Harvard

Khoshravesh, R & Sage, TL 2018, Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features. in S Covshoff (ed.), Photosynthesis. Methods in Molecular Biology, vol. 1770, Humana Press, New York, pp. 253-261. https://doi.org/10.1007/978-1-4939-7786-4_15

APA

Khoshravesh, R., & Sage, T. L. (2018). Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features. In S. Covshoff (Ed.), Photosynthesis (pp. 253-261). (Methods in Molecular Biology; Vol. 1770). Humana Press. https://doi.org/10.1007/978-1-4939-7786-4_15

Vancouver

Khoshravesh R, Sage TL. Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features. In Covshoff S, editor, Photosynthesis. New York: Humana Press. 2018. p. 253-261. (Methods in Molecular Biology). Epub 2018 Jul 6. doi: 10.1007/978-1-4939-7786-4_15

Author

Khoshravesh, Roxana ; Sage, Tammy L. / Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features. Photosynthesis. editor / S. Covshoff. New York : Humana Press, 2018. pp. 253-261 (Methods in Molecular Biology).

Bibtex

@inbook{52d2c9d2cde14356bc639935f42d4938,

title = "Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features",

abstract = "Imaging of mesophyll cell suspensions prepared from Arabidopsis has been pivotal for forming our current understanding of the molecular control of chloroplast division over the past 25 years. In this chapter, we provide a method for the preparation of leaf cell suspensions that improves upon a previous method by optimizing cellular preservation and cell separation. This technique is accessible to all researchers and amenable for use with all plant species. The leaf suspensions can be used for imaging chloroplast features within a cell that are important for photosynthesis such as size, number, and distribution. However, we also provide examples to illustrate how the cells in the suspensions can be easily stained to image other features, for example pit fields where plasmodesmata are located and organelles such as mitochondria, to improve our understanding of traits that are important for photosynthetic physiology.",

keywords = "Cell Culture Techniques, Chloroplasts/metabolism, Mesophyll Cells/metabolism, Microscopy, Fluorescence, Plant Leaves/cytology, Plant Vascular Bundle/cytology",

author = "Roxana Khoshravesh and Sage, {Tammy L}",

year = "2018",

month = jul,

day = "30",

doi = "10.1007/978-1-4939-7786-4_15",

language = "English",

isbn = "9781493977857",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "253--261",

editor = "S. Covshoff",

booktitle = "Photosynthesis",

}

RIS

TY - CHAP

T1 - Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features

AU - Khoshravesh, Roxana

AU - Sage, Tammy L

PY - 2018/7/30

Y1 - 2018/7/30

N2 - Imaging of mesophyll cell suspensions prepared from Arabidopsis has been pivotal for forming our current understanding of the molecular control of chloroplast division over the past 25 years. In this chapter, we provide a method for the preparation of leaf cell suspensions that improves upon a previous method by optimizing cellular preservation and cell separation. This technique is accessible to all researchers and amenable for use with all plant species. The leaf suspensions can be used for imaging chloroplast features within a cell that are important for photosynthesis such as size, number, and distribution. However, we also provide examples to illustrate how the cells in the suspensions can be easily stained to image other features, for example pit fields where plasmodesmata are located and organelles such as mitochondria, to improve our understanding of traits that are important for photosynthetic physiology.

AB - Imaging of mesophyll cell suspensions prepared from Arabidopsis has been pivotal for forming our current understanding of the molecular control of chloroplast division over the past 25 years. In this chapter, we provide a method for the preparation of leaf cell suspensions that improves upon a previous method by optimizing cellular preservation and cell separation. This technique is accessible to all researchers and amenable for use with all plant species. The leaf suspensions can be used for imaging chloroplast features within a cell that are important for photosynthesis such as size, number, and distribution. However, we also provide examples to illustrate how the cells in the suspensions can be easily stained to image other features, for example pit fields where plasmodesmata are located and organelles such as mitochondria, to improve our understanding of traits that are important for photosynthetic physiology.

KW - Cell Culture Techniques

KW - Chloroplasts/metabolism

KW - Mesophyll Cells/metabolism

KW - Microscopy, Fluorescence

KW - Plant Leaves/cytology

KW - Plant Vascular Bundle/cytology

U2 - 10.1007/978-1-4939-7786-4_15

DO - 10.1007/978-1-4939-7786-4_15

M3 - Chapter (peer-reviewed)

C2 - 29978407

SN - 9781493977857

T3 - Methods in Molecular Biology

SP - 253

EP - 261

BT - Photosynthesis

A2 - Covshoff, S.

PB - Humana Press

CY - New York

ER -

Research

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