Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Cytokinin producing bacteria stimulate amino acid deposition by wheat roots
AU - Kudoyarova, Guzel R.
AU - Melentiev, Alexander I.
AU - Martynenko, Elena V.
AU - Timergalina, Leila N.
AU - Arkhipova, Tatiana N.
AU - Shendel, Galina V.
AU - Kuz'mina, Ludmila Yu
AU - Dodd, Ian C.
AU - Veselov, Stanislav Yu
PY - 2014/10
Y1 - 2014/10
N2 - Phytohormone production is one mechanism by which rhizobacteria can stimulate plant growth, but it is not clear whether the bacteria gain from this mechanism. The hypothesis that microbial-derived cytokinin phytohormones stimulate root exudation of amino acids was tested. The rhizosphere of wheat plants was drenched with the synthetic cytokinin trans-zeatin or inoculated with Bacillus subtilis IB-22 (which produces zeatin type cytokinins) or B. subtilis IB-21 (which failed to accumulate cytokinins). Growing plants in a split root system allowed spatial separation of zeatin application or rhizobacterial inoculation to one compartment and analyses of amino acid release from roots (rhizodeposition) into the other compartment (without either microbial inoculation or treatment with exogenous hormone). Supplying B. sub tills IB-22 or zeatin to either the whole root system or half of the roots increased concentrations of amino acids in the soil solution although the magnitude of the increase was greater when whole roots were treated. There was some similarity in amino acid concentrations induced by either bacterial or zeatin treatment. Thus B. subtilis IB-22 increased amino acid rhizodeposition, likely due to its ability to produce cytokinins. Furthermore, B. subtilis strain IB-21, which failed to accumulate cytokinins in culture media, did not significantly affect amino acid concentrations in the wheat rhizosphere. The ability of rhizobacteria to produce cytokinins and thereby stimulate rhizodeposition may be important in enhancing rhizobacterial colonization of the rhizoplane. (C) 2014 Elsevier Masson SAS. All rights reserved.
AB - Phytohormone production is one mechanism by which rhizobacteria can stimulate plant growth, but it is not clear whether the bacteria gain from this mechanism. The hypothesis that microbial-derived cytokinin phytohormones stimulate root exudation of amino acids was tested. The rhizosphere of wheat plants was drenched with the synthetic cytokinin trans-zeatin or inoculated with Bacillus subtilis IB-22 (which produces zeatin type cytokinins) or B. subtilis IB-21 (which failed to accumulate cytokinins). Growing plants in a split root system allowed spatial separation of zeatin application or rhizobacterial inoculation to one compartment and analyses of amino acid release from roots (rhizodeposition) into the other compartment (without either microbial inoculation or treatment with exogenous hormone). Supplying B. sub tills IB-22 or zeatin to either the whole root system or half of the roots increased concentrations of amino acids in the soil solution although the magnitude of the increase was greater when whole roots were treated. There was some similarity in amino acid concentrations induced by either bacterial or zeatin treatment. Thus B. subtilis IB-22 increased amino acid rhizodeposition, likely due to its ability to produce cytokinins. Furthermore, B. subtilis strain IB-21, which failed to accumulate cytokinins in culture media, did not significantly affect amino acid concentrations in the wheat rhizosphere. The ability of rhizobacteria to produce cytokinins and thereby stimulate rhizodeposition may be important in enhancing rhizobacterial colonization of the rhizoplane. (C) 2014 Elsevier Masson SAS. All rights reserved.
KW - Bacillus subtilis
KW - Cytokinin
KW - Plant/microbe interaction
KW - Rhizodeposition
KW - Wheat
KW - GROWTH PROMOTING RHIZOBACTERIA
KW - PLANT-GROWTH
KW - BACILLUS-SUBTILIS
KW - RHIZOSPHERE
KW - SOIL
KW - EXPRESSION
KW - GENE
KW - RHIZODEPOSITION
KW - COLONIZATION
KW - EXUDATION
U2 - 10.1016/j.plaphy.2014.08.015
DO - 10.1016/j.plaphy.2014.08.015
M3 - Journal article
VL - 83
SP - 285
EP - 291
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
SN - 0981-9428
ER -