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Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis

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  • Michael Foster
  • William Brooks
  • Philipp Jahn
  • Jesper Hedberg
  • Andreas Andersson
  • Lorna Ashton
Article numbere202200021
<mark>Journal publication date</mark>31/07/2022
<mark>Journal</mark>Journal of Biophotonics
Issue number7
Number of pages8
Publication StatusPublished
Early online date3/05/22
<mark>Original language</mark>English


Monoclonal antibodies and antibody fragments are increasingly important classes of biotherapeutics. However, these products are both challenging and expensive to manufacture. New process analytical technologies used to monitor these products during their manufacture are of significant interest. Deep UV Raman spectroscopy promises to provide the required specificity and accuracy, however instruments, have historically been large and complex. In this paper, a new deep UV Raman instrument is described using a solid-state laser and a spatial heterodyne spectrometer. The instrument overcomes practical limitations of the technique and could readily be used for online measurement. A series of observations have been made of biopharmaceutical products, including immunoglobulin G and domain antibodies. Where high levels of both specificity and linearity when measuring samples of different concentration with a precision of better than 0.05 mg/mL has been demonstrated.