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Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis. / Foster, Michael; Brooks, William; Jahn, Philipp et al.
In: Journal of Biophotonics, Vol. 15, No. 7, e202200021, 31.07.2022.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Foster, M, Brooks, W, Jahn, P, Hedberg, J, Andersson, A & Ashton, L 2022, 'Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis', Journal of Biophotonics, vol. 15, no. 7, e202200021. https://doi.org/10.1002/jbio.202200021

APA

Foster, M., Brooks, W., Jahn, P., Hedberg, J., Andersson, A., & Ashton, L. (2022). Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis. Journal of Biophotonics, 15(7), Article e202200021. https://doi.org/10.1002/jbio.202200021

Vancouver

Foster M, Brooks W, Jahn P, Hedberg J, Andersson A, Ashton L. Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis. Journal of Biophotonics. 2022 Jul 31;15(7):e202200021. Epub 2022 May 3. doi: 10.1002/jbio.202200021

Author

Foster, Michael ; Brooks, William ; Jahn, Philipp et al. / Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis. In: Journal of Biophotonics. 2022 ; Vol. 15, No. 7.

Bibtex

@article{775c8dcd28de45eda65e51b128afb878,
title = "Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis",
abstract = "Monoclonal antibodies and antibody fragments are increasingly important classes of biotherapeutics. However, these products are both challenging and expensive to manufacture. New process analytical technologies used to monitor these products during their manufacture are of significant interest. Deep UV Raman spectroscopy promises to provide the required specificity and accuracy, however instruments, have historically been large and complex. In this paper, a new deep UV Raman instrument is described using a solid-state laser and a spatial heterodyne spectrometer. The instrument overcomes practical limitations of the technique and could readily be used for online measurement. A series of observations have been made of biopharmaceutical products, including immunoglobulin G and domain antibodies. Where high levels of both specificity and linearity when measuring samples of different concentration with a precision of better than 0.05 mg/mL has been demonstrated.",
keywords = "deep UV, deep UV Raman spectroscopy, monoclonal antibodies, PAT, proteins, Raman, resonant Raman spectroscopy",
author = "Michael Foster and William Brooks and Philipp Jahn and Jesper Hedberg and Andreas Andersson and Lorna Ashton",
year = "2022",
month = jul,
day = "31",
doi = "10.1002/jbio.202200021",
language = "English",
volume = "15",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley-VCH Verlag",
number = "7",

}

RIS

TY - JOUR

T1 - Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis

AU - Foster, Michael

AU - Brooks, William

AU - Jahn, Philipp

AU - Hedberg, Jesper

AU - Andersson, Andreas

AU - Ashton, Lorna

PY - 2022/7/31

Y1 - 2022/7/31

N2 - Monoclonal antibodies and antibody fragments are increasingly important classes of biotherapeutics. However, these products are both challenging and expensive to manufacture. New process analytical technologies used to monitor these products during their manufacture are of significant interest. Deep UV Raman spectroscopy promises to provide the required specificity and accuracy, however instruments, have historically been large and complex. In this paper, a new deep UV Raman instrument is described using a solid-state laser and a spatial heterodyne spectrometer. The instrument overcomes practical limitations of the technique and could readily be used for online measurement. A series of observations have been made of biopharmaceutical products, including immunoglobulin G and domain antibodies. Where high levels of both specificity and linearity when measuring samples of different concentration with a precision of better than 0.05 mg/mL has been demonstrated.

AB - Monoclonal antibodies and antibody fragments are increasingly important classes of biotherapeutics. However, these products are both challenging and expensive to manufacture. New process analytical technologies used to monitor these products during their manufacture are of significant interest. Deep UV Raman spectroscopy promises to provide the required specificity and accuracy, however instruments, have historically been large and complex. In this paper, a new deep UV Raman instrument is described using a solid-state laser and a spatial heterodyne spectrometer. The instrument overcomes practical limitations of the technique and could readily be used for online measurement. A series of observations have been made of biopharmaceutical products, including immunoglobulin G and domain antibodies. Where high levels of both specificity and linearity when measuring samples of different concentration with a precision of better than 0.05 mg/mL has been demonstrated.

KW - deep UV

KW - deep UV Raman spectroscopy

KW - monoclonal antibodies

KW - PAT

KW - proteins

KW - Raman

KW - resonant Raman spectroscopy

U2 - 10.1002/jbio.202200021

DO - 10.1002/jbio.202200021

M3 - Journal article

VL - 15

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 7

M1 - e202200021

ER -