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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis
AU - Foster, Michael
AU - Brooks, William
AU - Jahn, Philipp
AU - Hedberg, Jesper
AU - Andersson, Andreas
AU - Ashton, Lorna
PY - 2022/7/31
Y1 - 2022/7/31
N2 - Monoclonal antibodies and antibody fragments are increasingly important classes of biotherapeutics. However, these products are both challenging and expensive to manufacture. New process analytical technologies used to monitor these products during their manufacture are of significant interest. Deep UV Raman spectroscopy promises to provide the required specificity and accuracy, however instruments, have historically been large and complex. In this paper, a new deep UV Raman instrument is described using a solid-state laser and a spatial heterodyne spectrometer. The instrument overcomes practical limitations of the technique and could readily be used for online measurement. A series of observations have been made of biopharmaceutical products, including immunoglobulin G and domain antibodies. Where high levels of both specificity and linearity when measuring samples of different concentration with a precision of better than 0.05 mg/mL has been demonstrated.
AB - Monoclonal antibodies and antibody fragments are increasingly important classes of biotherapeutics. However, these products are both challenging and expensive to manufacture. New process analytical technologies used to monitor these products during their manufacture are of significant interest. Deep UV Raman spectroscopy promises to provide the required specificity and accuracy, however instruments, have historically been large and complex. In this paper, a new deep UV Raman instrument is described using a solid-state laser and a spatial heterodyne spectrometer. The instrument overcomes practical limitations of the technique and could readily be used for online measurement. A series of observations have been made of biopharmaceutical products, including immunoglobulin G and domain antibodies. Where high levels of both specificity and linearity when measuring samples of different concentration with a precision of better than 0.05 mg/mL has been demonstrated.
KW - deep UV
KW - deep UV Raman spectroscopy
KW - monoclonal antibodies
KW - PAT
KW - proteins
KW - Raman
KW - resonant Raman spectroscopy
U2 - 10.1002/jbio.202200021
DO - 10.1002/jbio.202200021
M3 - Journal article
VL - 15
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 7
M1 - e202200021
ER -