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Detection, distribution and probable fate of Escherichia coli 0157 from asymptomatic cattle on a dairy farm

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Detection, distribution and probable fate of Escherichia coli 0157 from asymptomatic cattle on a dairy farm. / Porter, J.; Mobbs, K.; Hart, C. A. et al.
In: Journal of Applied Microbiology, Vol. 83, No. 3, 01.08.1997, p. 297-306.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Porter, J, Mobbs, K, Hart, CA, Saunders, JR, Pickup, RW & Edwards, C 1997, 'Detection, distribution and probable fate of Escherichia coli 0157 from asymptomatic cattle on a dairy farm', Journal of Applied Microbiology, vol. 83, no. 3, pp. 297-306. https://doi.org/10.1046/j.1365-2672.1997.00230.x

APA

Porter, J., Mobbs, K., Hart, C. A., Saunders, J. R., Pickup, R. W., & Edwards, C. (1997). Detection, distribution and probable fate of Escherichia coli 0157 from asymptomatic cattle on a dairy farm. Journal of Applied Microbiology, 83(3), 297-306. https://doi.org/10.1046/j.1365-2672.1997.00230.x

Vancouver

Porter J, Mobbs K, Hart CA, Saunders JR, Pickup RW, Edwards C. Detection, distribution and probable fate of Escherichia coli 0157 from asymptomatic cattle on a dairy farm. Journal of Applied Microbiology. 1997 Aug 1;83(3):297-306. doi: 10.1046/j.1365-2672.1997.00230.x

Author

Porter, J. ; Mobbs, K. ; Hart, C. A. et al. / Detection, distribution and probable fate of Escherichia coli 0157 from asymptomatic cattle on a dairy farm. In: Journal of Applied Microbiology. 1997 ; Vol. 83, No. 3. pp. 297-306.

Bibtex

@article{6bf1bb6f0ed74dfca65090092ef14432,
title = "Detection, distribution and probable fate of Escherichia coli 0157 from asymptomatic cattle on a dairy farm",
abstract = "The use of commercial anti-Escherichia coli 0157-labelled magnetic beads was investigated to improve detection of E. coli 0157 by immunomagnetic separation (IMS) from a range of environments on a dairy farm. Immunomagnetic separation proved effective for separation of target cells from laboratory mixtures and during stress in sterile and non-sterile pond water. The IMS procedure was possible with a range of samples (water, faeces, slurry, grass and soil). Non-specific binding of non-target bacterial cells proved problematic in a number of sample types. However, indigenous E. coli 0157 cells were detected from samples with a high faecal load, and only with use of IMS. Data on the probable survival and spread of the organism around the farm environment are also discussed.",
author = "J. Porter and K. Mobbs and Hart, {C. A.} and Saunders, {J. R.} and Pickup, {R. W.} and C. Edwards",
year = "1997",
month = aug,
day = "1",
doi = "10.1046/j.1365-2672.1997.00230.x",
language = "English",
volume = "83",
pages = "297--306",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Detection, distribution and probable fate of Escherichia coli 0157 from asymptomatic cattle on a dairy farm

AU - Porter, J.

AU - Mobbs, K.

AU - Hart, C. A.

AU - Saunders, J. R.

AU - Pickup, R. W.

AU - Edwards, C.

PY - 1997/8/1

Y1 - 1997/8/1

N2 - The use of commercial anti-Escherichia coli 0157-labelled magnetic beads was investigated to improve detection of E. coli 0157 by immunomagnetic separation (IMS) from a range of environments on a dairy farm. Immunomagnetic separation proved effective for separation of target cells from laboratory mixtures and during stress in sterile and non-sterile pond water. The IMS procedure was possible with a range of samples (water, faeces, slurry, grass and soil). Non-specific binding of non-target bacterial cells proved problematic in a number of sample types. However, indigenous E. coli 0157 cells were detected from samples with a high faecal load, and only with use of IMS. Data on the probable survival and spread of the organism around the farm environment are also discussed.

AB - The use of commercial anti-Escherichia coli 0157-labelled magnetic beads was investigated to improve detection of E. coli 0157 by immunomagnetic separation (IMS) from a range of environments on a dairy farm. Immunomagnetic separation proved effective for separation of target cells from laboratory mixtures and during stress in sterile and non-sterile pond water. The IMS procedure was possible with a range of samples (water, faeces, slurry, grass and soil). Non-specific binding of non-target bacterial cells proved problematic in a number of sample types. However, indigenous E. coli 0157 cells were detected from samples with a high faecal load, and only with use of IMS. Data on the probable survival and spread of the organism around the farm environment are also discussed.

U2 - 10.1046/j.1365-2672.1997.00230.x

DO - 10.1046/j.1365-2672.1997.00230.x

M3 - Journal article

C2 - 9351209

AN - SCOPUS:0030876357

VL - 83

SP - 297

EP - 306

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 3

ER -