Home > Research > Publications & Outputs > Development of a real-time qPCR assay for quant...

Links

Text available via DOI:

View graph of relations

Development of a real-time qPCR assay for quantification of covert baculovirus infections in a major african crop pest

Research output: Contribution to journalJournal articlepeer-review

Published

Standard

Development of a real-time qPCR assay for quantification of covert baculovirus infections in a major african crop pest. / Graham, Robert Iain; Tummala Munuswamy, Yamini; Rhodes, Glenn; Cory, Jenny S.; Shirras, Alan Duncan; Grzywacz, David; Wilson, Kenneth.

In: Insects, Vol. 6, No. 3, 25.08.2015, p. 746-759.

Research output: Contribution to journalJournal articlepeer-review

Harvard

APA

Vancouver

Author

Bibtex

@article{59155d3b932d4b8e96cf92c51c8dbe69,
title = "Development of a real-time qPCR assay for quantification of covert baculovirus infections in a major african crop pest",
abstract = "Many pathogens and parasites are present in host individuals and populations without any obvious signs of disease. This is particularly true for baculoviruses infecting lepidopteran hosts, where studies have shown that covert persistent viral infections are almost ubiquitous in many species. To date, the infection intensity of covert viruses has rarely been quantified. In this study, we investigated the dynamics of a covert baculovirus infection within the lepidopteran crop pest Spodoptera exempta. A real-time quantitative polymerase chain reaction (qPCR) procedure using a 5' nuclease hydrolysis (TaqMan) probe was developed for specific detection and quantification of Spodoptera exempta nucleopolyhedrovirus (SpexNPV). The qPCR assay indicated that covert baculovirus dynamics varied considerably over the course of the host life-cycle, with infection load peaking in early larval instars and being lowest in adults and final-instar larvae. Adult dissections indicated that, contrary to expectation, viral load aggregation was highest in the head, wings and legs, and lowest in the thorax and abdomen. The data presented here have broad implications relating to our understanding of transmission patterns of baculoviruses and the role of covert infections in host-pathogen dynamics.",
keywords = "Spodoptera exempta, baculovirus, nucleopolyhedrovirus, covert infections, TaqMan real-time qPCR",
author = "Graham, {Robert Iain} and {Tummala Munuswamy}, Yamini and Glenn Rhodes and Cory, {Jenny S.} and Shirras, {Alan Duncan} and David Grzywacz and Kenneth Wilson",
year = "2015",
month = aug,
day = "25",
doi = "10.3390/insects6030746",
language = "English",
volume = "6",
pages = "746--759",
journal = "Insects",
issn = "2075-4450",
publisher = "MDPI AG",
number = "3",

}

RIS

TY - JOUR

T1 - Development of a real-time qPCR assay for quantification of covert baculovirus infections in a major african crop pest

AU - Graham, Robert Iain

AU - Tummala Munuswamy, Yamini

AU - Rhodes, Glenn

AU - Cory, Jenny S.

AU - Shirras, Alan Duncan

AU - Grzywacz, David

AU - Wilson, Kenneth

PY - 2015/8/25

Y1 - 2015/8/25

N2 - Many pathogens and parasites are present in host individuals and populations without any obvious signs of disease. This is particularly true for baculoviruses infecting lepidopteran hosts, where studies have shown that covert persistent viral infections are almost ubiquitous in many species. To date, the infection intensity of covert viruses has rarely been quantified. In this study, we investigated the dynamics of a covert baculovirus infection within the lepidopteran crop pest Spodoptera exempta. A real-time quantitative polymerase chain reaction (qPCR) procedure using a 5' nuclease hydrolysis (TaqMan) probe was developed for specific detection and quantification of Spodoptera exempta nucleopolyhedrovirus (SpexNPV). The qPCR assay indicated that covert baculovirus dynamics varied considerably over the course of the host life-cycle, with infection load peaking in early larval instars and being lowest in adults and final-instar larvae. Adult dissections indicated that, contrary to expectation, viral load aggregation was highest in the head, wings and legs, and lowest in the thorax and abdomen. The data presented here have broad implications relating to our understanding of transmission patterns of baculoviruses and the role of covert infections in host-pathogen dynamics.

AB - Many pathogens and parasites are present in host individuals and populations without any obvious signs of disease. This is particularly true for baculoviruses infecting lepidopteran hosts, where studies have shown that covert persistent viral infections are almost ubiquitous in many species. To date, the infection intensity of covert viruses has rarely been quantified. In this study, we investigated the dynamics of a covert baculovirus infection within the lepidopteran crop pest Spodoptera exempta. A real-time quantitative polymerase chain reaction (qPCR) procedure using a 5' nuclease hydrolysis (TaqMan) probe was developed for specific detection and quantification of Spodoptera exempta nucleopolyhedrovirus (SpexNPV). The qPCR assay indicated that covert baculovirus dynamics varied considerably over the course of the host life-cycle, with infection load peaking in early larval instars and being lowest in adults and final-instar larvae. Adult dissections indicated that, contrary to expectation, viral load aggregation was highest in the head, wings and legs, and lowest in the thorax and abdomen. The data presented here have broad implications relating to our understanding of transmission patterns of baculoviruses and the role of covert infections in host-pathogen dynamics.

KW - Spodoptera exempta

KW - baculovirus

KW - nucleopolyhedrovirus

KW - covert infections

KW - TaqMan real-time qPCR

U2 - 10.3390/insects6030746

DO - 10.3390/insects6030746

M3 - Journal article

VL - 6

SP - 746

EP - 759

JO - Insects

JF - Insects

SN - 2075-4450

IS - 3

ER -