Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Development of K-doped ZnO nanoparticles encapsulated crosslinked chitosan based new membranes to stimulate angiogenesis in tissue engineered skin grafts
AU - Shahzadi, Lubna
AU - Chaudhry, Aqif Anwar
AU - Aleem, Abdur Raheem
AU - Malik, Muhammad Hamza
AU - Ijaz, Kashif
AU - Akhtar, Hafsah
AU - Alvi, Farah
AU - Khan, Ather Farooq
AU - Rehman, Ihtesham Ur
AU - Yar, Muhammad
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Nanoparticles are well recognized for their biological applications including tissue-regeneration due to large surface area and chemical properties. In this study, K-doped zinc oxide (ZnO) nanoparticles containing porous hydrogels were synthesized via freeze gelation. The morphology and pore dimensions were studied by scanning electron microscopy (SEM). The chemical structural analysis of the synthesized hydrogels was investigated by Fourier Transform Infrared (FTIR) spectroscopy. In swelling studies, material containing ZnO nanoparticles with 2% potassium dopant concentration CLH-K2.0) showed greater degree of swelling as compared to all other materials. The degradation studied was tested in three different degradation media, i.e. phosphate buffer saline (PBS), lysozyme and hydrogen peroxide and relatively higher degradation was seen in hydrogen peroxide. The synthesized hydrogels were implanted on the chick chorioallantoic membrane (CAM) to investigate their angiogenic potential. The CLH-K2.0 hydrogel stimulated angiogenesis greater than all other materials; blood vessels were attached and grown inside this scaffold, showing its strong angiogenic potential.
AB - Nanoparticles are well recognized for their biological applications including tissue-regeneration due to large surface area and chemical properties. In this study, K-doped zinc oxide (ZnO) nanoparticles containing porous hydrogels were synthesized via freeze gelation. The morphology and pore dimensions were studied by scanning electron microscopy (SEM). The chemical structural analysis of the synthesized hydrogels was investigated by Fourier Transform Infrared (FTIR) spectroscopy. In swelling studies, material containing ZnO nanoparticles with 2% potassium dopant concentration CLH-K2.0) showed greater degree of swelling as compared to all other materials. The degradation studied was tested in three different degradation media, i.e. phosphate buffer saline (PBS), lysozyme and hydrogen peroxide and relatively higher degradation was seen in hydrogen peroxide. The synthesized hydrogels were implanted on the chick chorioallantoic membrane (CAM) to investigate their angiogenic potential. The CLH-K2.0 hydrogel stimulated angiogenesis greater than all other materials; blood vessels were attached and grown inside this scaffold, showing its strong angiogenic potential.
KW - K-doped ZnO nanoparticles
KW - Freeze-gelation
KW - Biodegradable hydrogels
KW - Angiogenesis
KW - Blood vascularization
U2 - 10.1016/j.ijbiomac.2018.08.103
DO - 10.1016/j.ijbiomac.2018.08.103
M3 - Journal article
VL - 120
SP - 721
EP - 728
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
SN - 0141-8130
ER -