Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Direct analysis of starved Aeromonas salmonicida
AU - Deere, D.
AU - Porter, J.
AU - Pickup, R.
AU - Edwards, C.
PY - 1996/11/30
Y1 - 1996/11/30
N2 - Survival of Aeromonas salmonicida was monitored during prolonged incubation in either distilled water or lake water. Culturability was determined from colony forming units enumerated on tryptone soy agar, whilst flow cytometry was used for direct analysis of viable cells after staining with fluorescent dyes which differentially stained bacteria in relation to defined cellular properties. Over time, populations of culturable cells steadily declined and were not detected after 10 days incubation in distilled water or 33 days in lake water. Flow cytometric analysis revealed that cellular properties related to viability were lost shortly after culturable cells became undetectable in distilled water. In contrast, those incubated in lake water showed little change in these properties over a 57-day experimental period. The implications of these differences are discussed, and it is concluded that A. salmonicida is capable of remaining intact and active upon prolonged incubation in lake water, although this does not conclusively prove viability.
AB - Survival of Aeromonas salmonicida was monitored during prolonged incubation in either distilled water or lake water. Culturability was determined from colony forming units enumerated on tryptone soy agar, whilst flow cytometry was used for direct analysis of viable cells after staining with fluorescent dyes which differentially stained bacteria in relation to defined cellular properties. Over time, populations of culturable cells steadily declined and were not detected after 10 days incubation in distilled water or 33 days in lake water. Flow cytometric analysis revealed that cellular properties related to viability were lost shortly after culturable cells became undetectable in distilled water. In contrast, those incubated in lake water showed little change in these properties over a 57-day experimental period. The implications of these differences are discussed, and it is concluded that A. salmonicida is capable of remaining intact and active upon prolonged incubation in lake water, although this does not conclusively prove viability.
U2 - 10.1111/j.1365-2761.1996.tb00386.x
DO - 10.1111/j.1365-2761.1996.tb00386.x
M3 - Journal article
AN - SCOPUS:0030483626
VL - 19
SP - 459
EP - 467
JO - Journal of Fish Diseases
JF - Journal of Fish Diseases
SN - 0140-7775
IS - 6
ER -