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Directional PC12 Cell Migration Along Plastic Nanotracks

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Directional PC12 Cell Migration Along Plastic Nanotracks. / Ferrari, Aldo; Cecchini, Marco; Beltram, Fabio et al.
In: IEEE Transactions on Biomedical Engineering, Vol. 56, No. 11, 2009, p. 2692-2696.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Ferrari, A, Cecchini, M, Beltram, F & Degl'Innocenti, R 2009, 'Directional PC12 Cell Migration Along Plastic Nanotracks', IEEE Transactions on Biomedical Engineering, vol. 56, no. 11, pp. 2692-2696. https://doi.org/10.1109/TBME.2009.2027424

APA

Ferrari, A., Cecchini, M., Beltram, F., & Degl'Innocenti, R. (2009). Directional PC12 Cell Migration Along Plastic Nanotracks. IEEE Transactions on Biomedical Engineering, 56(11), 2692-2696. https://doi.org/10.1109/TBME.2009.2027424

Vancouver

Ferrari A, Cecchini M, Beltram F, Degl'Innocenti R. Directional PC12 Cell Migration Along Plastic Nanotracks. IEEE Transactions on Biomedical Engineering. 2009;56(11):2692-2696. doi: 10.1109/TBME.2009.2027424

Author

Ferrari, Aldo ; Cecchini, Marco ; Beltram, Fabio et al. / Directional PC12 Cell Migration Along Plastic Nanotracks. In: IEEE Transactions on Biomedical Engineering. 2009 ; Vol. 56, No. 11. pp. 2692-2696.

Bibtex

@article{fdd3a77185bb49c3811e2e265173dd9b,
title = "Directional PC12 Cell Migration Along Plastic Nanotracks",
abstract = "The design of materials to promote the development and/or regeneration of neuronal tissue requires the understanding of the mechanisms by which the underlying substrate topography can modulate neuronal cell differentiation and migration. We recently demonstrated that plastic nanogratings (alternating lines of grooves and ridges of submicrometer size) can effectively change the neuronal polarity state, selecting bipolar cells with aligned neu-rites. Here, we address the effect of nanogratings on the migration properties of differentiating PC12 cells and correlate their behavior with the polarity state induced by the substrate. During neuronal differentiation, cell-substrate interaction is sufficient to induce directional migration along the nanogratings. Control cells contacting flat substrates migrated freely in all directions, while cells differentiating on nanogratings showed slower migration characterized by an angular restriction that confined cell movements. Finally, we show that directional migration on nanogratings is linked to a specific organization of the cell cytoskeleton reflecting the nanograting directionality.",
keywords = "Contact guidance, neuronal migration, nucleokinesis, topography",
author = "Aldo Ferrari and Marco Cecchini and Fabio Beltram and Riccardo Degl'Innocenti",
year = "2009",
doi = "10.1109/TBME.2009.2027424",
language = "English",
volume = "56",
pages = "2692--2696",
journal = "IEEE Transactions on Biomedical Engineering",
issn = "0018-9294",
publisher = "IEEE Computer Society",
number = "11",

}

RIS

TY - JOUR

T1 - Directional PC12 Cell Migration Along Plastic Nanotracks

AU - Ferrari, Aldo

AU - Cecchini, Marco

AU - Beltram, Fabio

AU - Degl'Innocenti, Riccardo

PY - 2009

Y1 - 2009

N2 - The design of materials to promote the development and/or regeneration of neuronal tissue requires the understanding of the mechanisms by which the underlying substrate topography can modulate neuronal cell differentiation and migration. We recently demonstrated that plastic nanogratings (alternating lines of grooves and ridges of submicrometer size) can effectively change the neuronal polarity state, selecting bipolar cells with aligned neu-rites. Here, we address the effect of nanogratings on the migration properties of differentiating PC12 cells and correlate their behavior with the polarity state induced by the substrate. During neuronal differentiation, cell-substrate interaction is sufficient to induce directional migration along the nanogratings. Control cells contacting flat substrates migrated freely in all directions, while cells differentiating on nanogratings showed slower migration characterized by an angular restriction that confined cell movements. Finally, we show that directional migration on nanogratings is linked to a specific organization of the cell cytoskeleton reflecting the nanograting directionality.

AB - The design of materials to promote the development and/or regeneration of neuronal tissue requires the understanding of the mechanisms by which the underlying substrate topography can modulate neuronal cell differentiation and migration. We recently demonstrated that plastic nanogratings (alternating lines of grooves and ridges of submicrometer size) can effectively change the neuronal polarity state, selecting bipolar cells with aligned neu-rites. Here, we address the effect of nanogratings on the migration properties of differentiating PC12 cells and correlate their behavior with the polarity state induced by the substrate. During neuronal differentiation, cell-substrate interaction is sufficient to induce directional migration along the nanogratings. Control cells contacting flat substrates migrated freely in all directions, while cells differentiating on nanogratings showed slower migration characterized by an angular restriction that confined cell movements. Finally, we show that directional migration on nanogratings is linked to a specific organization of the cell cytoskeleton reflecting the nanograting directionality.

KW - Contact guidance

KW - neuronal migration

KW - nucleokinesis

KW - topography

U2 - 10.1109/TBME.2009.2027424

DO - 10.1109/TBME.2009.2027424

M3 - Journal article

C2 - 19643702

AN - SCOPUS:74049122595

VL - 56

SP - 2692

EP - 2696

JO - IEEE Transactions on Biomedical Engineering

JF - IEEE Transactions on Biomedical Engineering

SN - 0018-9294

IS - 11

ER -