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Distinct regulation of ATM signaling by DNA single-strand breaks and APE1

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Distinct regulation of ATM signaling by DNA single-strand breaks and APE1. / Zhao, Haichao; Li, Jia ; You, Zhongsheng et al.
In: Nature Communications, Vol. 15, 6517, 07.08.2024.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Zhao, H, Li, J, You, Z, Lindsay, H & Yan, S 2024, 'Distinct regulation of ATM signaling by DNA single-strand breaks and APE1', Nature Communications, vol. 15, 6517. https://doi.org/10.1038/s41467-024-50836-6

APA

Zhao, H., Li, J., You, Z., Lindsay, H., & Yan, S. (2024). Distinct regulation of ATM signaling by DNA single-strand breaks and APE1. Nature Communications, 15, Article 6517. https://doi.org/10.1038/s41467-024-50836-6

Vancouver

Zhao H, Li J, You Z, Lindsay H, Yan S. Distinct regulation of ATM signaling by DNA single-strand breaks and APE1. Nature Communications. 2024 Aug 7;15:6517. doi: 10.1038/s41467-024-50836-6

Author

Zhao, Haichao ; Li, Jia ; You, Zhongsheng et al. / Distinct regulation of ATM signaling by DNA single-strand breaks and APE1. In: Nature Communications. 2024 ; Vol. 15.

Bibtex

@article{861c83e961c94d9a8119458494387caa,
title = "Distinct regulation of ATM signaling by DNA single-strand breaks and APE1",
abstract = "In response to DNA double-strand breaks or oxidative stress, ATM-dependentDNA damage response (DDR) is activated to maintain genome integrity.However, it remains elusive whether and how DNA single-strand breaks (SSBs)activate ATM. Here, we provide direct evidence in Xenopus egg extracts thatATM-mediated DDR is activated by a defined SSB structure. Our mechanisticstudies reveal that APE1 promotes the SSB-induced ATM DDR through APE1exonuclease activity and ATM recruitment to SSB sites. APE1 protein can formoligomers to activate the ATM DDR in Xenopus egg extracts in the absence ofDNA and can directly stimulate ATM kinase activity in vitro. Our findings revealdistinct mechanisms of the ATM-dependent DDR activation by SSBs ineukaryotic systems and identify APE1 as a direct activator of ATM kinase.",
author = "Haichao Zhao and Jia Li and Zhongsheng You and Howard Lindsay and Shan Yan",
year = "2024",
month = aug,
day = "7",
doi = "10.1038/s41467-024-50836-6",
language = "English",
volume = "15",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Distinct regulation of ATM signaling by DNA single-strand breaks and APE1

AU - Zhao, Haichao

AU - Li, Jia

AU - You, Zhongsheng

AU - Lindsay, Howard

AU - Yan, Shan

PY - 2024/8/7

Y1 - 2024/8/7

N2 - In response to DNA double-strand breaks or oxidative stress, ATM-dependentDNA damage response (DDR) is activated to maintain genome integrity.However, it remains elusive whether and how DNA single-strand breaks (SSBs)activate ATM. Here, we provide direct evidence in Xenopus egg extracts thatATM-mediated DDR is activated by a defined SSB structure. Our mechanisticstudies reveal that APE1 promotes the SSB-induced ATM DDR through APE1exonuclease activity and ATM recruitment to SSB sites. APE1 protein can formoligomers to activate the ATM DDR in Xenopus egg extracts in the absence ofDNA and can directly stimulate ATM kinase activity in vitro. Our findings revealdistinct mechanisms of the ATM-dependent DDR activation by SSBs ineukaryotic systems and identify APE1 as a direct activator of ATM kinase.

AB - In response to DNA double-strand breaks or oxidative stress, ATM-dependentDNA damage response (DDR) is activated to maintain genome integrity.However, it remains elusive whether and how DNA single-strand breaks (SSBs)activate ATM. Here, we provide direct evidence in Xenopus egg extracts thatATM-mediated DDR is activated by a defined SSB structure. Our mechanisticstudies reveal that APE1 promotes the SSB-induced ATM DDR through APE1exonuclease activity and ATM recruitment to SSB sites. APE1 protein can formoligomers to activate the ATM DDR in Xenopus egg extracts in the absence ofDNA and can directly stimulate ATM kinase activity in vitro. Our findings revealdistinct mechanisms of the ATM-dependent DDR activation by SSBs ineukaryotic systems and identify APE1 as a direct activator of ATM kinase.

U2 - 10.1038/s41467-024-50836-6

DO - 10.1038/s41467-024-50836-6

M3 - Journal article

VL - 15

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 6517

ER -