Home > Research > Publications & Outputs > E. coli SbcCD and RecA control chromosomal rear...

Research

Links

Text available via DOI:

Keywords

View graph of relations

E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome. / Darmon, Elise; Eykelenboom, John K; Lincker, Frédéric et al.
In: Molecular Cell, Vol. 39, No. 1, 09.07.2010, p. 59-70.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Darmon, E, Eykelenboom, JK, Lincker, F, Jones, LH, White, M, Okely, E, Blackwood, JK & Leach, DR 2010, 'E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome', Molecular Cell, vol. 39, no. 1, pp. 59-70. https://doi.org/10.1016/j.molcel.2010.06.011

APA

Darmon, E., Eykelenboom, J. K., Lincker, F., Jones, L. H., White, M., Okely, E., Blackwood, J. K., & Leach, D. R. (2010). E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome. Molecular Cell, 39(1), 59-70. https://doi.org/10.1016/j.molcel.2010.06.011

Vancouver

Darmon E, Eykelenboom JK, Lincker F, Jones LH, White M, Okely E et al. E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome. Molecular Cell. 2010 Jul 9;39(1):59-70. Epub 2010 Jul 8. doi: 10.1016/j.molcel.2010.06.011

Author

Darmon, Elise ; Eykelenboom, John K ; Lincker, Frédéric et al. / E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome. In: Molecular Cell. 2010 ; Vol. 39, No. 1. pp. 59-70.

Bibtex

@article{d4884b78f73749a69e31aa9eccd7bfd7,
title = "E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome",
abstract = "Survival and genome stability are critical characteristics of healthy cells. DNA palindromes pose a threat to genome stability and have been shown to participate in a reaction leading to the formation of inverted chromosome duplications centered around themselves. There is considerable interest in the mechanism of this rearrangement given its likely contribution to genome instability in cancer cells. This study shows that formation of large inverted chromosome duplications can be observed in the chromosome of Escherichia coli. They are formed at the site of a 246 bp interrupted DNA palindrome in the absence of the hairpin nuclease SbcCD and the recombination protein RecA. The genetic requirements for this spontaneous rearrangement are consistent with a pathway involving DNA degradation and hairpin formation, as opposed to a cruciform cleavage pathway. Accordingly, the formation of palindrome-dependent hairpin intermediates can be induced by an adjacent DNA double-stand break.",
keywords = "Base Pairing, Chromosomes, Bacterial, DNA Breaks, Double-Stranded, DNA Replication, DNA, Bacterial, Deoxyribonucleases, Escherichia coli, Escherichia coli Proteins, Exonucleases, Gene Rearrangement, Inverted Repeat Sequences, Microscopy, Models, Biological, Rec A Recombinases, Recombination, Genetic, Journal Article, Research Support, Non-U.S. Gov't",
author = "Elise Darmon and Eykelenboom, {John K} and Fr{\'e}d{\'e}ric Lincker and Jones, {Lucy H} and Martin White and Ewa Okely and Blackwood, {John K} and Leach, {David R}",
note = "2010 Elsevier Inc. All rights reserved.",
year = "2010",
month = jul,
day = "9",
doi = "10.1016/j.molcel.2010.06.011",
language = "English",
volume = "39",
pages = "59--70",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - E. coli SbcCD and RecA control chromosomal rearrangement induced by an interrupted palindrome

AU - Darmon, Elise

AU - Eykelenboom, John K

AU - Lincker, Frédéric

AU - Jones, Lucy H

AU - White, Martin

AU - Okely, Ewa

AU - Blackwood, John K

AU - Leach, David R

N1 - 2010 Elsevier Inc. All rights reserved.

PY - 2010/7/9

Y1 - 2010/7/9

N2 - Survival and genome stability are critical characteristics of healthy cells. DNA palindromes pose a threat to genome stability and have been shown to participate in a reaction leading to the formation of inverted chromosome duplications centered around themselves. There is considerable interest in the mechanism of this rearrangement given its likely contribution to genome instability in cancer cells. This study shows that formation of large inverted chromosome duplications can be observed in the chromosome of Escherichia coli. They are formed at the site of a 246 bp interrupted DNA palindrome in the absence of the hairpin nuclease SbcCD and the recombination protein RecA. The genetic requirements for this spontaneous rearrangement are consistent with a pathway involving DNA degradation and hairpin formation, as opposed to a cruciform cleavage pathway. Accordingly, the formation of palindrome-dependent hairpin intermediates can be induced by an adjacent DNA double-stand break.

AB - Survival and genome stability are critical characteristics of healthy cells. DNA palindromes pose a threat to genome stability and have been shown to participate in a reaction leading to the formation of inverted chromosome duplications centered around themselves. There is considerable interest in the mechanism of this rearrangement given its likely contribution to genome instability in cancer cells. This study shows that formation of large inverted chromosome duplications can be observed in the chromosome of Escherichia coli. They are formed at the site of a 246 bp interrupted DNA palindrome in the absence of the hairpin nuclease SbcCD and the recombination protein RecA. The genetic requirements for this spontaneous rearrangement are consistent with a pathway involving DNA degradation and hairpin formation, as opposed to a cruciform cleavage pathway. Accordingly, the formation of palindrome-dependent hairpin intermediates can be induced by an adjacent DNA double-stand break.

KW - Base Pairing

KW - Chromosomes, Bacterial

KW - DNA Breaks, Double-Stranded

KW - DNA Replication

KW - DNA, Bacterial

KW - Deoxyribonucleases

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Exonucleases

KW - Gene Rearrangement

KW - Inverted Repeat Sequences

KW - Microscopy

KW - Models, Biological

KW - Rec A Recombinases

KW - Recombination, Genetic

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.molcel.2010.06.011

DO - 10.1016/j.molcel.2010.06.011

M3 - Journal article

C2 - 20603075

VL - 39

SP - 59

EP - 70

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 1

ER -