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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Efficacy of Disinfectants against Egyptian H5N1 Avian Influenza Virus
AU - Rohaim, Mohammed
AU - El Naggar, Rania
AU - Gamal, Abdulrahman M.
AU - Ismael, Elshaimaa
AU - Hamoud, Mohamed M.
AU - Moubarak, Sherif T.
AU - Metwally, Ashraf M.
AU - Zaki, Manal M.
AU - Nasr, Shimaa A.E.
AU - Elsaid, Samah
AU - Ali, Mohamed M.
AU - Hussein, Hussein A.
AU - Zahran, Osama K.
PY - 2015/10/31
Y1 - 2015/10/31
N2 - Poultry industry in Egypt is facing various management problems along with infectious diseases including avian influenza (AI). Biosecurity measures, controlling poultry movements and inactivated vaccines were devised to combat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highlysusceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly reduce and/or limit the chance for its transmission and outbreaks. Many disinfectants have been evaluated for their inactivation ability, but there is still a need for their evaluation under different conditions and in different ways. In the present study, representative disinfectants from chlorine and non-chlorine oxidizing agents have been evaluated for their virucidal ability against two distinct Egyptiansubclades of H5N1 highly pathogenic avian influenza (HPAI); (A/chicken/Egypt/VRLCU67/2011) variantsubclade 2.2.1.1 and (A/chicken/Egypt/13VIR3729-4/2013) classic subclade 2.2.1/C that were sodiumhypochlorite, calcium hypochlorite (bleaching powder), Virkon® S, and Peraclean®. The purpose from usingthe Egyptian H5N1 viruses in the evaluation was to achieve maximum simulation of Egyptian field realityas the two viruses represent the two main subclades currently co-circulating in Egypt. The disinfectants weretested individually for effectiveness against HPAI H5N1 for 5, 10, 15 and 30 minutes contact time. Numericalmethod and neutralization test were used to assess the ability of each disinfectant to inactivate the virus. Ourresults revealed that all the used disinfectants were effective with increasing the contact time more than 15minutes except with Virkon® S which was effective even at a short contact time, 5 minutes. In conclusion, thisstudy reported that chlorine and non-chlorine oxidizing agents are effective against H5N1 HPAI at the farmlevel that would be helpful in implementing bio-security measures at farms/hatcheries levels in the wake ofavian influenza virus (AIV) outbreak.
AB - Poultry industry in Egypt is facing various management problems along with infectious diseases including avian influenza (AI). Biosecurity measures, controlling poultry movements and inactivated vaccines were devised to combat the spread of highly pathogenic avian influenza (HPAIV) H5N1. HPAIV are highlysusceptible to all disinfectants because they are enveloped viruses. Disinfection against avian influenza viruses at the poultry farms would significantly reduce and/or limit the chance for its transmission and outbreaks. Many disinfectants have been evaluated for their inactivation ability, but there is still a need for their evaluation under different conditions and in different ways. In the present study, representative disinfectants from chlorine and non-chlorine oxidizing agents have been evaluated for their virucidal ability against two distinct Egyptiansubclades of H5N1 highly pathogenic avian influenza (HPAI); (A/chicken/Egypt/VRLCU67/2011) variantsubclade 2.2.1.1 and (A/chicken/Egypt/13VIR3729-4/2013) classic subclade 2.2.1/C that were sodiumhypochlorite, calcium hypochlorite (bleaching powder), Virkon® S, and Peraclean®. The purpose from usingthe Egyptian H5N1 viruses in the evaluation was to achieve maximum simulation of Egyptian field realityas the two viruses represent the two main subclades currently co-circulating in Egypt. The disinfectants weretested individually for effectiveness against HPAI H5N1 for 5, 10, 15 and 30 minutes contact time. Numericalmethod and neutralization test were used to assess the ability of each disinfectant to inactivate the virus. Ourresults revealed that all the used disinfectants were effective with increasing the contact time more than 15minutes except with Virkon® S which was effective even at a short contact time, 5 minutes. In conclusion, thisstudy reported that chlorine and non-chlorine oxidizing agents are effective against H5N1 HPAI at the farmlevel that would be helpful in implementing bio-security measures at farms/hatcheries levels in the wake ofavian influenza virus (AIV) outbreak.
M3 - Journal article
VL - 2
SP - 80
EP - 87
JO - British Journal of Virology
JF - British Journal of Virology
SN - 2055-6128
IS - 5
ER -