Home > Research > Publications & Outputs > Electrochemical detection of plasma immunoglobu...


Text available via DOI:

View graph of relations

Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease

Research output: Contribution to Journal/MagazineJournal articlepeer-review

  • Goulielmos Zois Garyfallou
  • Orlando Ketebu
  • Samet Şahin
  • Elizabeta B. Mukaetova-Ladinska
  • Michael Catt
  • Eileen Hao Yu
Article number2464
<mark>Journal publication date</mark>27/10/2017
<mark>Journal</mark>Sensors (Switzerland)
Issue number11
Publication StatusPublished
<mark>Original language</mark>English


The clinical diagnosis and treatment of Alzheimer’s disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 109 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL1 range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices.