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Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease

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Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease. / Garyfallou, Goulielmos Zois; Ketebu, Orlando; Şahin, Samet et al.
In: Sensors (Switzerland), Vol. 17, No. 11, 2464, 27.10.2017.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Garyfallou, GZ, Ketebu, O, Şahin, S, Mukaetova-Ladinska, EB, Catt, M & Yu, EH 2017, 'Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease', Sensors (Switzerland), vol. 17, no. 11, 2464. https://doi.org/10.3390/s17112464

APA

Garyfallou, G. Z., Ketebu, O., Şahin, S., Mukaetova-Ladinska, E. B., Catt, M., & Yu, E. H. (2017). Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease. Sensors (Switzerland), 17(11), Article 2464. https://doi.org/10.3390/s17112464

Vancouver

Garyfallou GZ, Ketebu O, Şahin S, Mukaetova-Ladinska EB, Catt M, Yu EH. Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease. Sensors (Switzerland). 2017 Oct 27;17(11):2464. doi: 10.3390/s17112464

Author

Garyfallou, Goulielmos Zois ; Ketebu, Orlando ; Şahin, Samet et al. / Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease. In: Sensors (Switzerland). 2017 ; Vol. 17, No. 11.

Bibtex

@article{ecf4f621b53e45e1b484b9b1f8b9e5e5,
title = "Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer{\textquoteright}s disease",
abstract = "The clinical diagnosis and treatment of Alzheimer{\textquoteright}s disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 10−9 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL−1 range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices.",
keywords = "Alzheimer{\textquoteright}s disease, Direct immunosensor, Electrochemical impedance spectroscopy, Plasma immunoglobulin, Polyclonal rabbit Anti-human immunoglobulin",
author = "Garyfallou, {Goulielmos Zois} and Orlando Ketebu and Samet {\c S}ahin and Mukaetova-Ladinska, {Elizabeta B.} and Michael Catt and Yu, {Eileen Hao}",
year = "2017",
month = oct,
day = "27",
doi = "10.3390/s17112464",
language = "English",
volume = "17",
journal = "Sensors (Switzerland)",
issn = "1424-8220",
publisher = "NLM (Medline)",
number = "11",

}

RIS

TY - JOUR

T1 - Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease

AU - Garyfallou, Goulielmos Zois

AU - Ketebu, Orlando

AU - Şahin, Samet

AU - Mukaetova-Ladinska, Elizabeta B.

AU - Catt, Michael

AU - Yu, Eileen Hao

PY - 2017/10/27

Y1 - 2017/10/27

N2 - The clinical diagnosis and treatment of Alzheimer’s disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 10−9 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL−1 range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices.

AB - The clinical diagnosis and treatment of Alzheimer’s disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 10−9 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL−1 range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices.

KW - Alzheimer’s disease

KW - Direct immunosensor

KW - Electrochemical impedance spectroscopy

KW - Plasma immunoglobulin

KW - Polyclonal rabbit Anti-human immunoglobulin

U2 - 10.3390/s17112464

DO - 10.3390/s17112464

M3 - Journal article

C2 - 29077013

AN - SCOPUS:85032656527

VL - 17

JO - Sensors (Switzerland)

JF - Sensors (Switzerland)

SN - 1424-8220

IS - 11

M1 - 2464

ER -