Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Electrochemical detection of plasma immunoglobulin as a biomarker for Alzheimer’s disease
AU - Garyfallou, Goulielmos Zois
AU - Ketebu, Orlando
AU - Şahin, Samet
AU - Mukaetova-Ladinska, Elizabeta B.
AU - Catt, Michael
AU - Yu, Eileen Hao
PY - 2017/10/27
Y1 - 2017/10/27
N2 - The clinical diagnosis and treatment of Alzheimer’s disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 10−9 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL−1 range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices.
AB - The clinical diagnosis and treatment of Alzheimer’s disease (AD) represent a challenge to clinicians due to the variability of clinical symptomatology as well as the unavailability of reliable diagnostic tests. In this study, the development of a novel electrochemical assay and its potential to detect peripheral blood biomarkers to diagnose AD using plasma immunoglobulins is investigated. The immunosensor employs a gold electrode as the immobilizing substrate, albumin depleted plasma immunoglobulin as the biomarker, and polyclonal rabbit Anti-human immunoglobulin (against IgA, IgG, IgM) as the receptor for plasma conjugation. The assay showed good response, sensitivity and reproducibility in differentiating plasma immunoglobulin from AD and control subjects down to 10−9 dilutions of plasma immunoglobulin representing plasma content concentrations in the pg mL−1 range. The newly developed assay is highly sensitive, less time consuming, easy to handle, can be easily modified to detect other dementia-related biomarkers in blood samples, and can be easily integrated into portable devices.
KW - Alzheimer’s disease
KW - Direct immunosensor
KW - Electrochemical impedance spectroscopy
KW - Plasma immunoglobulin
KW - Polyclonal rabbit Anti-human immunoglobulin
U2 - 10.3390/s17112464
DO - 10.3390/s17112464
M3 - Journal article
C2 - 29077013
AN - SCOPUS:85032656527
VL - 17
JO - Sensors (Switzerland)
JF - Sensors (Switzerland)
SN - 1424-8220
IS - 11
M1 - 2464
ER -