Home > Research > Publications & Outputs > Elevated CO2 improved the growth of a double ni...

Electronic data

  • 1-s2.0-S0098847217301363-main

    Rights statement: This is the author’s version of a work that was accepted for publication in Environmental and Experimental Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental and Experimental Botany, 140, 2017 DOI: 10.1016/j.envexpbot.2017.06.003

    Accepted author manuscript, 959 KB, PDF document

    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Links

Text available via DOI:

View graph of relations

Elevated CO2 improved the growth of a double nitrate reductase defective mutant of Arabidopsis thaliana: the importance of maintaining a high energy status

Research output: Contribution to journalJournal articlepeer-review

Published
  • Ivan Jauregui
  • Pedro M. Aparicio-Tejo
  • Edurne Baroja
  • Concepción Avila
  • Iker Aranjuelo
Close
<mark>Journal publication date</mark>08/2017
<mark>Journal</mark>Environmental and Experimental Botany
Volume140
Number of pages10
Pages (from-to)110-119
Publication StatusPublished
Early online date9/06/17
<mark>Original language</mark>English

Abstract

Impairments in leaf nitrogen (N) assimilation in C3 plants have been identified as processes conditioning photosynthesis under elevated [CO2], especially when N is supplied as nitrate. Leaf N status is usually improved under ammonium nutrition and elevated [CO2]. However, ammonium fertilization is usually accompanied by the appearance of oxidative stress symptoms, which constrains plant development. To understand how the limitations of direct fertilization with ammonium (growth reduction attributed to ammonium toxicity) can be overcome, the effects of elevated [CO2] (800 ppm) exposure were studied in the Arabidopsis thaliana double nitrate reductase defective mutant, nia1-1/chl3-5 (which preferentially assimilates ammonium as its nitrogen source). Analysis of the physiology, metabolites and gene expression was carried out in roots and shoot organs. Our study clearly showed that elevated [CO2] improved the inhibited phenotype of the nitrate reductase double mutant. Both the photosynthetic rates and the leaf N content of the NR mutant under elevated CO2 were similar to wild type plants. The growth of the nitrate reductase mutant was linked to its ability to overcome ammonium-associated photoinhibition processes at 800 ppm [CO2]. More specifically: (i) the capacity of NR mutants to equilibrate energy availability, as reflected by the electron transport equilibrium reached (photosynthesis, photorespiration and respiration), (ii) as well as by the upregulation of genes involved in stress tolerance were identified as the processes involved in the improved performance of NR mutants.

Bibliographic note

This is the author’s version of a work that was accepted for publication in Environmental and Experimental Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Environmental and Experimental Botany, 140, 2017 DOI: 10.1016/j.envexpbot.2017.06.003