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En-face analysis of the human limbal lymphatic vasculature

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En-face analysis of the human limbal lymphatic vasculature. / Palme, Christoph; Ahmad, Sajjad; Romano, Vito et al.
In: Experimental Eye Research, Vol. 201, 108278, 31.12.2020.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Palme, C, Ahmad, S, Romano, V, Seifarth, C, Williams, B, Parekh, M, Kaye, SB & Steger, B 2020, 'En-face analysis of the human limbal lymphatic vasculature', Experimental Eye Research, vol. 201, 108278. https://doi.org/10.1016/j.exer.2020.108278

APA

Palme, C., Ahmad, S., Romano, V., Seifarth, C., Williams, B., Parekh, M., Kaye, S. B., & Steger, B. (2020). En-face analysis of the human limbal lymphatic vasculature. Experimental Eye Research, 201, Article 108278. https://doi.org/10.1016/j.exer.2020.108278

Vancouver

Palme C, Ahmad S, Romano V, Seifarth C, Williams B, Parekh M et al. En-face analysis of the human limbal lymphatic vasculature. Experimental Eye Research. 2020 Dec 31;201:108278. Epub 2020 Sept 28. doi: 10.1016/j.exer.2020.108278

Author

Palme, Christoph ; Ahmad, Sajjad ; Romano, Vito et al. / En-face analysis of the human limbal lymphatic vasculature. In: Experimental Eye Research. 2020 ; Vol. 201.

Bibtex

@article{c705107cfe9049fab265c4997d0ae10e,
title = "En-face analysis of the human limbal lymphatic vasculature",
abstract = "Purpose: To describe the location and morphometric characteristics of the human limbal lymphatic vasculature and its relation to the marginal corneal vascular arcades (MCA). Methods: Ex vivo confocal microscopic (CM) imaging and immunofluorescence double staining for CD-31 and D2-40 of histological en-face sections using 12 preserved human cadaveric corneoscleral discs were performed, followed by a semi-automated morphometric analysis of the two-dimensional vascular network architecture. Results: Ex vivo CM confirmed the presence of 2 distinct vascular networks. The haematic limbal vascular complex (HLVC) extended further into the cornea, forming typical MCAs. The lymphatic limbal vascular complex (LLVC) was peripheral from the termination of Bowman's layer and was also found to be peripheral to and deeper than the HLVC. LLVC and HLVC were significantly different with respect to vessel diameter, segment length and wall thickness. Conclusion: The lymphatic vasculature of the human corneoscleral limbal region displays specific morphometric features that allow its differentiation from haematic vessels using CM.",
keywords = "Confocal microscopy, Corneoscleral limbus, Immunofluorescence, Lymphatic vessels",
author = "Christoph Palme and Sajjad Ahmad and Vito Romano and Christof Seifarth and Bryan Williams and Mohit Parekh and Kaye, {Stephen B.} and Bernhard Steger",
year = "2020",
month = dec,
day = "31",
doi = "10.1016/j.exer.2020.108278",
language = "English",
volume = "201",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - En-face analysis of the human limbal lymphatic vasculature

AU - Palme, Christoph

AU - Ahmad, Sajjad

AU - Romano, Vito

AU - Seifarth, Christof

AU - Williams, Bryan

AU - Parekh, Mohit

AU - Kaye, Stephen B.

AU - Steger, Bernhard

PY - 2020/12/31

Y1 - 2020/12/31

N2 - Purpose: To describe the location and morphometric characteristics of the human limbal lymphatic vasculature and its relation to the marginal corneal vascular arcades (MCA). Methods: Ex vivo confocal microscopic (CM) imaging and immunofluorescence double staining for CD-31 and D2-40 of histological en-face sections using 12 preserved human cadaveric corneoscleral discs were performed, followed by a semi-automated morphometric analysis of the two-dimensional vascular network architecture. Results: Ex vivo CM confirmed the presence of 2 distinct vascular networks. The haematic limbal vascular complex (HLVC) extended further into the cornea, forming typical MCAs. The lymphatic limbal vascular complex (LLVC) was peripheral from the termination of Bowman's layer and was also found to be peripheral to and deeper than the HLVC. LLVC and HLVC were significantly different with respect to vessel diameter, segment length and wall thickness. Conclusion: The lymphatic vasculature of the human corneoscleral limbal region displays specific morphometric features that allow its differentiation from haematic vessels using CM.

AB - Purpose: To describe the location and morphometric characteristics of the human limbal lymphatic vasculature and its relation to the marginal corneal vascular arcades (MCA). Methods: Ex vivo confocal microscopic (CM) imaging and immunofluorescence double staining for CD-31 and D2-40 of histological en-face sections using 12 preserved human cadaveric corneoscleral discs were performed, followed by a semi-automated morphometric analysis of the two-dimensional vascular network architecture. Results: Ex vivo CM confirmed the presence of 2 distinct vascular networks. The haematic limbal vascular complex (HLVC) extended further into the cornea, forming typical MCAs. The lymphatic limbal vascular complex (LLVC) was peripheral from the termination of Bowman's layer and was also found to be peripheral to and deeper than the HLVC. LLVC and HLVC were significantly different with respect to vessel diameter, segment length and wall thickness. Conclusion: The lymphatic vasculature of the human corneoscleral limbal region displays specific morphometric features that allow its differentiation from haematic vessels using CM.

KW - Confocal microscopy

KW - Corneoscleral limbus

KW - Immunofluorescence

KW - Lymphatic vessels

U2 - 10.1016/j.exer.2020.108278

DO - 10.1016/j.exer.2020.108278

M3 - Journal article

C2 - 32997957

AN - SCOPUS:85091778102

VL - 201

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

M1 - 108278

ER -