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Epstein-Barr virus isolates retain their capacity to evade T cell immunity through BNLF2a despite extensive sequence variation

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Epstein-Barr virus isolates retain their capacity to evade T cell immunity through BNLF2a despite extensive sequence variation. / Horst, Daniëlle; Burrows, Scott R.; Gatherer, Derek et al.

In: Journal of Virology, Vol. 86, No. 1, 01.2012, p. 572-577.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Horst, D, Burrows, SR, Gatherer, D, van Wilgenburg, B, Boer, IGJ, Bell, MJ, Ressing, ME & Wiertz, EJHJ 2012, 'Epstein-Barr virus isolates retain their capacity to evade T cell immunity through BNLF2a despite extensive sequence variation', Journal of Virology, vol. 86, no. 1, pp. 572-577. https://doi.org/10.1128/JVI.05151-11

APA

Horst, D., Burrows, S. R., Gatherer, D., van Wilgenburg, B., Boer, I. G. J., Bell, M. J., Ressing, M. E., & Wiertz, E. J. H. J. (2012). Epstein-Barr virus isolates retain their capacity to evade T cell immunity through BNLF2a despite extensive sequence variation. Journal of Virology, 86(1), 572-577. https://doi.org/10.1128/JVI.05151-11

Vancouver

Horst D, Burrows SR, Gatherer D, van Wilgenburg B, Boer IGJ, Bell MJ et al. Epstein-Barr virus isolates retain their capacity to evade T cell immunity through BNLF2a despite extensive sequence variation. Journal of Virology. 2012 Jan;86(1):572-577. doi: 10.1128/JVI.05151-11

Author

Horst, Daniëlle ; Burrows, Scott R. ; Gatherer, Derek et al. / Epstein-Barr virus isolates retain their capacity to evade T cell immunity through BNLF2a despite extensive sequence variation. In: Journal of Virology. 2012 ; Vol. 86, No. 1. pp. 572-577.

Bibtex

@article{5370b01bbbbf4588bbd443e7510a25af,
title = "Epstein-Barr virus isolates retain their capacity to evade T cell immunity through BNLF2a despite extensive sequence variation",
abstract = "The Epstein-Barr virus (EBV)-encoded immune evasion protein BNLF2a inhibits the transporter associated with antigen processing (TAP), thereby downregulating HLA class I expression at the cell surface. As a consequence, recognition of EBV-infected cells by cytotoxic T cells is impaired. Here, we show that sequence polymorphism of the BNLF2a protein is observed with natural EBV isolates, with evidence for positive selection. Despite these mutations, the BNLF2a variants efficiently reduce cell surface HLA class I levels. This conservation of BNLF2a function during evolution of EBV implies an important role for the viral TAP inhibitor in preventing T cell recognition during viral infection.",
keywords = "ATP-Binding Cassette Transporters, Amino Acid Motifs, Amino Acid Sequence, Cell Line, Epstein-Barr Virus Infections, Evolution, Molecular, Genetic Variation, Herpesvirus 4, Human, Histocompatibility Antigens Class I, Humans, Immune Evasion, Molecular Sequence Data, Selection, Genetic, Sequence Alignment, T-Lymphocytes, Cytotoxic, Viral Matrix Proteins",
author = "Dani{\"e}lle Horst and Burrows, {Scott R.} and Derek Gatherer and {van Wilgenburg}, Bonnie and Boer, {Ingrid G. J.} and Bell, {Melissa J.} and Ressing, {Maaike E.} and Wiertz, {Emmanuel J. H. J.}",
year = "2012",
month = jan,
doi = "10.1128/JVI.05151-11",
language = "English",
volume = "86",
pages = "572--577",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

RIS

TY - JOUR

T1 - Epstein-Barr virus isolates retain their capacity to evade T cell immunity through BNLF2a despite extensive sequence variation

AU - Horst, Daniëlle

AU - Burrows, Scott R.

AU - Gatherer, Derek

AU - van Wilgenburg, Bonnie

AU - Boer, Ingrid G. J.

AU - Bell, Melissa J.

AU - Ressing, Maaike E.

AU - Wiertz, Emmanuel J. H. J.

PY - 2012/1

Y1 - 2012/1

N2 - The Epstein-Barr virus (EBV)-encoded immune evasion protein BNLF2a inhibits the transporter associated with antigen processing (TAP), thereby downregulating HLA class I expression at the cell surface. As a consequence, recognition of EBV-infected cells by cytotoxic T cells is impaired. Here, we show that sequence polymorphism of the BNLF2a protein is observed with natural EBV isolates, with evidence for positive selection. Despite these mutations, the BNLF2a variants efficiently reduce cell surface HLA class I levels. This conservation of BNLF2a function during evolution of EBV implies an important role for the viral TAP inhibitor in preventing T cell recognition during viral infection.

AB - The Epstein-Barr virus (EBV)-encoded immune evasion protein BNLF2a inhibits the transporter associated with antigen processing (TAP), thereby downregulating HLA class I expression at the cell surface. As a consequence, recognition of EBV-infected cells by cytotoxic T cells is impaired. Here, we show that sequence polymorphism of the BNLF2a protein is observed with natural EBV isolates, with evidence for positive selection. Despite these mutations, the BNLF2a variants efficiently reduce cell surface HLA class I levels. This conservation of BNLF2a function during evolution of EBV implies an important role for the viral TAP inhibitor in preventing T cell recognition during viral infection.

KW - ATP-Binding Cassette Transporters

KW - Amino Acid Motifs

KW - Amino Acid Sequence

KW - Cell Line

KW - Epstein-Barr Virus Infections

KW - Evolution, Molecular

KW - Genetic Variation

KW - Herpesvirus 4, Human

KW - Histocompatibility Antigens Class I

KW - Humans

KW - Immune Evasion

KW - Molecular Sequence Data

KW - Selection, Genetic

KW - Sequence Alignment

KW - T-Lymphocytes, Cytotoxic

KW - Viral Matrix Proteins

U2 - 10.1128/JVI.05151-11

DO - 10.1128/JVI.05151-11

M3 - Journal article

C2 - 22013037

VL - 86

SP - 572

EP - 577

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -