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Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway

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Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway. / Cholleti, Harindranath; Paidikondala, Maruthibabu; Munir, Muhammad et al.
In: Virus Research, Vol. 171, No. 1, 01.01.2013, p. 222-226.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Cholleti, H, Paidikondala, M, Munir, M, Hakhverdyan, M & Baule, C 2013, 'Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway', Virus Research, vol. 171, no. 1, pp. 222-226. https://doi.org/10.1016/j.virusres.2012.10.004

APA

Cholleti, H., Paidikondala, M., Munir, M., Hakhverdyan, M., & Baule, C. (2013). Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway. Virus Research, 171(1), 222-226. https://doi.org/10.1016/j.virusres.2012.10.004

Vancouver

Cholleti H, Paidikondala M, Munir M, Hakhverdyan M, Baule C. Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway. Virus Research. 2013 Jan 1;171(1):222-226. doi: 10.1016/j.virusres.2012.10.004

Author

Cholleti, Harindranath ; Paidikondala, Maruthibabu ; Munir, Muhammad et al. / Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway. In: Virus Research. 2013 ; Vol. 171, No. 1. pp. 222-226.

Bibtex

@article{bc170883aac241a0a8552e545b7ec97e,
title = "Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway",
abstract = "Equine arteritis virus (EAV) causes a respiratory and reproductive disease in horses, equine viral arteritis. Though cell death in infection with EAV is considered to occur by apoptosis, the underlying molecular mechanism has not been extensively elucidated. We investigated the expression of mRNA of pro-apoptotic and caspase genes during EAV infection in BHK21 cells, a well-established cell type for EAV replication. Using a SYBR Green real-time PCR, mRNA of p53, Bax, caspase 3 and caspase 9 were found up-regulated in a time dependent manner in EAV infected cells. Western blot analysis for caspase 3 and caspase 9 showed expression of cleaved forms of these proteins during EAV infection. In addition, a luminescence-based cell assay for caspase 3/7 activation as a hallmark in apoptosis confirmed apoptotic cell death. The findings demonstrate that cell death in EAV infected BHK21 cells results from apoptosis mediated through the intrinsic signalling pathway.",
keywords = "3/7, Apoptosis, Bax, Caspase 9, Equine arteritis virus, P53, Real-time PCR",
author = "Harindranath Cholleti and Maruthibabu Paidikondala and Muhammad Munir and Mikhayil Hakhverdyan and Claudia Baule",
year = "2013",
month = jan,
day = "1",
doi = "10.1016/j.virusres.2012.10.004",
language = "English",
volume = "171",
pages = "222--226",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway

AU - Cholleti, Harindranath

AU - Paidikondala, Maruthibabu

AU - Munir, Muhammad

AU - Hakhverdyan, Mikhayil

AU - Baule, Claudia

PY - 2013/1/1

Y1 - 2013/1/1

N2 - Equine arteritis virus (EAV) causes a respiratory and reproductive disease in horses, equine viral arteritis. Though cell death in infection with EAV is considered to occur by apoptosis, the underlying molecular mechanism has not been extensively elucidated. We investigated the expression of mRNA of pro-apoptotic and caspase genes during EAV infection in BHK21 cells, a well-established cell type for EAV replication. Using a SYBR Green real-time PCR, mRNA of p53, Bax, caspase 3 and caspase 9 were found up-regulated in a time dependent manner in EAV infected cells. Western blot analysis for caspase 3 and caspase 9 showed expression of cleaved forms of these proteins during EAV infection. In addition, a luminescence-based cell assay for caspase 3/7 activation as a hallmark in apoptosis confirmed apoptotic cell death. The findings demonstrate that cell death in EAV infected BHK21 cells results from apoptosis mediated through the intrinsic signalling pathway.

AB - Equine arteritis virus (EAV) causes a respiratory and reproductive disease in horses, equine viral arteritis. Though cell death in infection with EAV is considered to occur by apoptosis, the underlying molecular mechanism has not been extensively elucidated. We investigated the expression of mRNA of pro-apoptotic and caspase genes during EAV infection in BHK21 cells, a well-established cell type for EAV replication. Using a SYBR Green real-time PCR, mRNA of p53, Bax, caspase 3 and caspase 9 were found up-regulated in a time dependent manner in EAV infected cells. Western blot analysis for caspase 3 and caspase 9 showed expression of cleaved forms of these proteins during EAV infection. In addition, a luminescence-based cell assay for caspase 3/7 activation as a hallmark in apoptosis confirmed apoptotic cell death. The findings demonstrate that cell death in EAV infected BHK21 cells results from apoptosis mediated through the intrinsic signalling pathway.

KW - 3/7

KW - Apoptosis

KW - Bax

KW - Caspase 9

KW - Equine arteritis virus

KW - P53

KW - Real-time PCR

U2 - 10.1016/j.virusres.2012.10.004

DO - 10.1016/j.virusres.2012.10.004

M3 - Journal article

C2 - 23079113

AN - SCOPUS:84872824070

VL - 171

SP - 222

EP - 226

JO - Virus Research

JF - Virus Research

SN - 0168-1702

IS - 1

ER -