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Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid) trimethine oxonol, (diBA-C4(3) -), for the flow cytometric assessment of bacterial viability

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Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid) trimethine oxonol, (diBA-C4(3) -), for the flow cytometric assessment of bacterial viability. / Deere, D.; Porter, J.; Edwards, C. et al.
In: FEMS Microbiology Letters, Vol. 130, No. 2-3, 01.08.1995, p. 165-169.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Deere, D, Porter, J, Edwards, C & Pickup, R 1995, 'Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid) trimethine oxonol, (diBA-C4(3) -), for the flow cytometric assessment of bacterial viability', FEMS Microbiology Letters, vol. 130, no. 2-3, pp. 165-169. https://doi.org/10.1016/0378-1097(95)00199-F

APA

Deere, D., Porter, J., Edwards, C., & Pickup, R. (1995). Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid) trimethine oxonol, (diBA-C4(3) -), for the flow cytometric assessment of bacterial viability. FEMS Microbiology Letters, 130(2-3), 165-169. https://doi.org/10.1016/0378-1097(95)00199-F

Vancouver

Deere D, Porter J, Edwards C, Pickup R. Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid) trimethine oxonol, (diBA-C4(3) -), for the flow cytometric assessment of bacterial viability. FEMS Microbiology Letters. 1995 Aug 1;130(2-3):165-169. doi: 10.1016/0378-1097(95)00199-F

Author

Deere, D. ; Porter, J. ; Edwards, C. et al. / Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid) trimethine oxonol, (diBA-C4(3) -), for the flow cytometric assessment of bacterial viability. In: FEMS Microbiology Letters. 1995 ; Vol. 130, No. 2-3. pp. 165-169.

Bibtex

@article{d3b5810303d14b9a87b1a89ea7c54f35,
title = "Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid) trimethine oxonol, (diBA-C4(3) -), for the flow cytometric assessment of bacterial viability",
abstract = "The usefulness of oxonol (bis-(1,3-dibutylbarbituric acid)trimethine oxonol) as a generally applicable indicator of bacterial viability was investigated using untreated and killed cultures of a variety of bacterial genera. Killing methods involved either heat or bactericidal antibiotics. For all strains tested, the fluorescent dye showed significantly more intense staining of killed than untreated cells. The sensitivity of Aeromonas salmonicida to gentamicin was assessed using oxonol. Although the bacterium was shown to be sensitive to the antibiotic, there was a delay between the time cells lost culturability, as judged by numbers of colony forming units, and that for which a dead cell population could be detected by flow cytometry.",
keywords = "Bacterial viability, Flow cytometry, Membrane potential, Oxonol dye",
author = "D. Deere and J. Porter and C. Edwards and R. Pickup",
year = "1995",
month = aug,
day = "1",
doi = "10.1016/0378-1097(95)00199-F",
language = "English",
volume = "130",
pages = "165--169",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2-3",

}

RIS

TY - JOUR

T1 - Evaluation of the suitability of bis-(1,3-dibutylbarbituric acid) trimethine oxonol, (diBA-C4(3) -), for the flow cytometric assessment of bacterial viability

AU - Deere, D.

AU - Porter, J.

AU - Edwards, C.

AU - Pickup, R.

PY - 1995/8/1

Y1 - 1995/8/1

N2 - The usefulness of oxonol (bis-(1,3-dibutylbarbituric acid)trimethine oxonol) as a generally applicable indicator of bacterial viability was investigated using untreated and killed cultures of a variety of bacterial genera. Killing methods involved either heat or bactericidal antibiotics. For all strains tested, the fluorescent dye showed significantly more intense staining of killed than untreated cells. The sensitivity of Aeromonas salmonicida to gentamicin was assessed using oxonol. Although the bacterium was shown to be sensitive to the antibiotic, there was a delay between the time cells lost culturability, as judged by numbers of colony forming units, and that for which a dead cell population could be detected by flow cytometry.

AB - The usefulness of oxonol (bis-(1,3-dibutylbarbituric acid)trimethine oxonol) as a generally applicable indicator of bacterial viability was investigated using untreated and killed cultures of a variety of bacterial genera. Killing methods involved either heat or bactericidal antibiotics. For all strains tested, the fluorescent dye showed significantly more intense staining of killed than untreated cells. The sensitivity of Aeromonas salmonicida to gentamicin was assessed using oxonol. Although the bacterium was shown to be sensitive to the antibiotic, there was a delay between the time cells lost culturability, as judged by numbers of colony forming units, and that for which a dead cell population could be detected by flow cytometry.

KW - Bacterial viability

KW - Flow cytometry

KW - Membrane potential

KW - Oxonol dye

U2 - 10.1016/0378-1097(95)00199-F

DO - 10.1016/0378-1097(95)00199-F

M3 - Journal article

C2 - 7649437

AN - SCOPUS:0028878032

VL - 130

SP - 165

EP - 169

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2-3

ER -