Home > Research > Publications & Outputs > Fucose content of keratan sulphates from bovine...
View graph of relations

Fucose content of keratan sulphates from bovine articular cartilage

Research output: Contribution to Journal/MagazineJournal articlepeer-review

<mark>Journal publication date</mark>1991
<mark>Journal</mark>Biochemical Journal
Issue number2
Number of pages4
Pages (from-to)307-310
Publication StatusPublished
<mark>Original language</mark>English


Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6-8-year-old animals). Nine keratan sulphate fractions of increasing molecular weight were prepared by gel-permeation chromatography on a calibrated column of TSK 30 XL. The samples were analysed for fucose and galactose contents (% by wt. of keratan sulphate) and fucose/galactose ratio. The fucose content increased with molecular size, but the galactose content remained constant. It was concluded that the alpha(1----3)-linked fucose [Thornton, Morris, Cockin, Huckerby, Nieduszynski, Carlstedt, Hardingham & Ratcliffe (1989) Biochem. J. 260, 277-282] was located within the poly-N-acetyl-lactosamine repeat sequence of articular-cartilage keratan sulphate.