Fucose content of keratan sulphates from bovine articular cartilage

Biomedical and Life Sciences

Keywords

Animals, Borohydrides, Carbohydrates, Cartilage, Articular, Cattle, Chromatography, Gel, Fucose, Galactose, Keratan Sulfate, Magnetic Resonance Spectroscopy, Molecular Weight

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Fucose content of keratan sulphates from bovine articular cartilage. / Tai, G H; Brown, G M; Morris, H G et al.
In: Biochemical Journal, Vol. 273, No. 2, 1991, p. 307-310.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Author

Tai, G H ; Brown, G M ; Morris, H G et al. / Fucose content of keratan sulphates from bovine articular cartilage. In: Biochemical Journal. 1991 ; Vol. 273, No. 2. pp. 307-310.

Bibtex

@article{ea1d6dc8b0c34c67805b46199dd51711,

title = "Fucose content of keratan sulphates from bovine articular cartilage",

abstract = "Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6-8-year-old animals). Nine keratan sulphate fractions of increasing molecular weight were prepared by gel-permeation chromatography on a calibrated column of TSK 30 XL. The samples were analysed for fucose and galactose contents (% by wt. of keratan sulphate) and fucose/galactose ratio. The fucose content increased with molecular size, but the galactose content remained constant. It was concluded that the alpha(1----3)-linked fucose [Thornton, Morris, Cockin, Huckerby, Nieduszynski, Carlstedt, Hardingham & Ratcliffe (1989) Biochem. J. 260, 277-282] was located within the poly-N-acetyl-lactosamine repeat sequence of articular-cartilage keratan sulphate.",

keywords = "Animals, Borohydrides, Carbohydrates, Cartilage, Articular, Cattle, Chromatography, Gel, Fucose, Galactose, Keratan Sulfate, Magnetic Resonance Spectroscopy, Molecular Weight",

author = "Tai, {G H} and Brown, {G M} and Morris, {H G} and Huckerby, {T N} and Nieduszynski, {I A}",

year = "1991",

language = "English",

volume = "273",

pages = "307--310",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

number = "2",

}

RIS

TY - JOUR

T1 - Fucose content of keratan sulphates from bovine articular cartilage

AU - Tai, G H

AU - Brown, G M

AU - Morris, H G

AU - Huckerby, T N

AU - Nieduszynski, I A

PY - 1991

Y1 - 1991

N2 - Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6-8-year-old animals). Nine keratan sulphate fractions of increasing molecular weight were prepared by gel-permeation chromatography on a calibrated column of TSK 30 XL. The samples were analysed for fucose and galactose contents (% by wt. of keratan sulphate) and fucose/galactose ratio. The fucose content increased with molecular size, but the galactose content remained constant. It was concluded that the alpha(1----3)-linked fucose [Thornton, Morris, Cockin, Huckerby, Nieduszynski, Carlstedt, Hardingham & Ratcliffe (1989) Biochem. J. 260, 277-282] was located within the poly-N-acetyl-lactosamine repeat sequence of articular-cartilage keratan sulphate.

AB - Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6-8-year-old animals). Nine keratan sulphate fractions of increasing molecular weight were prepared by gel-permeation chromatography on a calibrated column of TSK 30 XL. The samples were analysed for fucose and galactose contents (% by wt. of keratan sulphate) and fucose/galactose ratio. The fucose content increased with molecular size, but the galactose content remained constant. It was concluded that the alpha(1----3)-linked fucose [Thornton, Morris, Cockin, Huckerby, Nieduszynski, Carlstedt, Hardingham & Ratcliffe (1989) Biochem. J. 260, 277-282] was located within the poly-N-acetyl-lactosamine repeat sequence of articular-cartilage keratan sulphate.

KW - Animals

KW - Borohydrides

KW - Carbohydrates

KW - Cartilage, Articular

KW - Cattle

KW - Chromatography, Gel

KW - Fucose

KW - Galactose

KW - Keratan Sulfate

KW - Magnetic Resonance Spectroscopy

KW - Molecular Weight

M3 - Journal article

C2 - 1991030

VL - 273

SP - 307

EP - 310

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 2

ER -

Research

Links

Keywords