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GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis. / Zhang, Yuhua; Wang, Yifei; Kanyuka, Kostya et al.

In: Journal of Experimental Botany, Vol. 59, No. 11, 08.2008, p. 3131-3141.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Zhang, Y, Wang, Y, Kanyuka, K, Parry, MAJ, Powers, SJ & Halford, NG 2008, 'GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis', Journal of Experimental Botany, vol. 59, no. 11, pp. 3131-3141. https://doi.org/10.1093/jxb/ern169

APA

Zhang, Y., Wang, Y., Kanyuka, K., Parry, M. A. J., Powers, S. J., & Halford, N. G. (2008). GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis. Journal of Experimental Botany, 59(11), 3131-3141. https://doi.org/10.1093/jxb/ern169

Vancouver

Zhang Y, Wang Y, Kanyuka K, Parry MAJ, Powers SJ, Halford NG. GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis. Journal of Experimental Botany. 2008 Aug;59(11):3131-3141. Epub 2008 Jul 4. doi: 10.1093/jxb/ern169

Author

Zhang, Yuhua ; Wang, Yifei ; Kanyuka, Kostya et al. / GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis. In: Journal of Experimental Botany. 2008 ; Vol. 59, No. 11. pp. 3131-3141.

Bibtex

@article{4dc60017e60d474ab1592a54e50dfd23,
title = "GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis",
abstract = "The yeast regulatory protein kinase, general control non-derepressible-2 (GCN2) plays a key role in general amino acid control. GCN2 phosphorylates the α subunit of the trimeric eukaryotic translation initiation factor-2 (eIF2), bringing about a decrease in the general rate of protein synthesis but an increase in the synthesis of GCN4, a transcription factor that promotes the expression of genes encoding enzymes for amino acid biosynthesis. The present study concerned the phosphorylation of Arabidopsis eIF2α (AteIF2α) by the Arabidopsis homologue of GCN2, AtGCN2, and the role of AtGCN2 in regulating genes encoding enzymes of amino acid biosynthesis and responding to virus infection. A null mutant for AtGCN2 called GT8359 was obtained and western analysis confirmed that it lacked AtGCN2 protein. GT8359 was more sensitive than wild-type Arabidopsis to herbicides that affect amino acid biosynthesis. Phosphorylation of AteIF2α occurred in response to herbicide treatment but only in wild-type Arabidopsis, not GT8359, showing it to be AtGCN2-dependent. Expression analysis of genes encoding key enzymes for amino acid biosynthesis and nitrate assimilation revealed little effect of loss of AtGCN2 function in GT8359 except that expression of a nitrate reductase gene, NIA1, was decreased. Analysis of wild-type and GT8359 plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2α was not phosphorylated.",
keywords = "Amino acid signalling, General control non-derepressible, Metabolic regulation, Phosphorylation, Protein kinase, Virus infection",
author = "Yuhua Zhang and Yifei Wang and Kostya Kanyuka and Parry, {Martin A. J.} and Powers, {Stephen J.} and Halford, {Nigel G.}",
year = "2008",
month = aug,
doi = "10.1093/jxb/ern169",
language = "English",
volume = "59",
pages = "3131--3141",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "OXFORD UNIV PRESS",
number = "11",

}

RIS

TY - JOUR

T1 - GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis

AU - Zhang, Yuhua

AU - Wang, Yifei

AU - Kanyuka, Kostya

AU - Parry, Martin A. J.

AU - Powers, Stephen J.

AU - Halford, Nigel G.

PY - 2008/8

Y1 - 2008/8

N2 - The yeast regulatory protein kinase, general control non-derepressible-2 (GCN2) plays a key role in general amino acid control. GCN2 phosphorylates the α subunit of the trimeric eukaryotic translation initiation factor-2 (eIF2), bringing about a decrease in the general rate of protein synthesis but an increase in the synthesis of GCN4, a transcription factor that promotes the expression of genes encoding enzymes for amino acid biosynthesis. The present study concerned the phosphorylation of Arabidopsis eIF2α (AteIF2α) by the Arabidopsis homologue of GCN2, AtGCN2, and the role of AtGCN2 in regulating genes encoding enzymes of amino acid biosynthesis and responding to virus infection. A null mutant for AtGCN2 called GT8359 was obtained and western analysis confirmed that it lacked AtGCN2 protein. GT8359 was more sensitive than wild-type Arabidopsis to herbicides that affect amino acid biosynthesis. Phosphorylation of AteIF2α occurred in response to herbicide treatment but only in wild-type Arabidopsis, not GT8359, showing it to be AtGCN2-dependent. Expression analysis of genes encoding key enzymes for amino acid biosynthesis and nitrate assimilation revealed little effect of loss of AtGCN2 function in GT8359 except that expression of a nitrate reductase gene, NIA1, was decreased. Analysis of wild-type and GT8359 plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2α was not phosphorylated.

AB - The yeast regulatory protein kinase, general control non-derepressible-2 (GCN2) plays a key role in general amino acid control. GCN2 phosphorylates the α subunit of the trimeric eukaryotic translation initiation factor-2 (eIF2), bringing about a decrease in the general rate of protein synthesis but an increase in the synthesis of GCN4, a transcription factor that promotes the expression of genes encoding enzymes for amino acid biosynthesis. The present study concerned the phosphorylation of Arabidopsis eIF2α (AteIF2α) by the Arabidopsis homologue of GCN2, AtGCN2, and the role of AtGCN2 in regulating genes encoding enzymes of amino acid biosynthesis and responding to virus infection. A null mutant for AtGCN2 called GT8359 was obtained and western analysis confirmed that it lacked AtGCN2 protein. GT8359 was more sensitive than wild-type Arabidopsis to herbicides that affect amino acid biosynthesis. Phosphorylation of AteIF2α occurred in response to herbicide treatment but only in wild-type Arabidopsis, not GT8359, showing it to be AtGCN2-dependent. Expression analysis of genes encoding key enzymes for amino acid biosynthesis and nitrate assimilation revealed little effect of loss of AtGCN2 function in GT8359 except that expression of a nitrate reductase gene, NIA1, was decreased. Analysis of wild-type and GT8359 plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2α was not phosphorylated.

KW - Amino acid signalling

KW - General control non-derepressible

KW - Metabolic regulation

KW - Phosphorylation

KW - Protein kinase

KW - Virus infection

U2 - 10.1093/jxb/ern169

DO - 10.1093/jxb/ern169

M3 - Journal article

C2 - 18603615

AN - SCOPUS:49449103129

VL - 59

SP - 3131

EP - 3141

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 11

ER -