Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Genetic analysis of Newcastle disease virus from Punjab, Pakistan
AU - Shabbir, Muhammad Zubair
AU - Abbas, Muhammad
AU - Yaqub, Tahir
AU - Mukhtar, Nadia
AU - Subhani, Atta
AU - Habib, Hasham
AU - Sohail, Muhammad Umar
AU - Munir, Muhammad
PY - 2013/4/1
Y1 - 2013/4/1
N2 - The strains of Newcastle disease virus (NDV) were isolated from five suspected outbreaks of ND in broiler (n = 3) and layers (n = 2) poultry farms. The egg-isolated viruses were subjected to biological and genetic characterization. Based on the biological characterization, isolates showed haemagglutination titer ≥log 27, mean death time <55 h, intracerebral pathogenecity index ≤1.8, and egg lethal dose 50 from 10 -7.15 to 10-9.31/1 ml. Genetic characterization of the fusion (F) gene revealed that the isolates clustered with NDV strains of genotype VII (VIIf) within class II, which remained predominant genotype in the domestic poultry of Asia. The deduced amino acid sequence of the isolates confirmed virulent motif 112RRQKRF117 at the F protein cleavage site. A bioinformatics and pairwise comparison approach was applied to estimate the synonymous and non-synonymous substitution rates (dN/dS) and selective evolutionary pressure for the F protein. The dN/dS calculated for genotype VII indicate purifying selection, which resulted in a low evolution rate in F gene. The F protein shows a strong negative pressure throughout the length of the protein and no recombination event was determined. Collectively, the results suggest that very similar virulent strains of NDV are involved during current wave of disease outbreak throughout the country. From these results, in conjunction with our recent reports of NDV from Pakistan, it is possible to conclude that emergence of novel group may require revisiting the diagnostics and vaccine control strategies.
AB - The strains of Newcastle disease virus (NDV) were isolated from five suspected outbreaks of ND in broiler (n = 3) and layers (n = 2) poultry farms. The egg-isolated viruses were subjected to biological and genetic characterization. Based on the biological characterization, isolates showed haemagglutination titer ≥log 27, mean death time <55 h, intracerebral pathogenecity index ≤1.8, and egg lethal dose 50 from 10 -7.15 to 10-9.31/1 ml. Genetic characterization of the fusion (F) gene revealed that the isolates clustered with NDV strains of genotype VII (VIIf) within class II, which remained predominant genotype in the domestic poultry of Asia. The deduced amino acid sequence of the isolates confirmed virulent motif 112RRQKRF117 at the F protein cleavage site. A bioinformatics and pairwise comparison approach was applied to estimate the synonymous and non-synonymous substitution rates (dN/dS) and selective evolutionary pressure for the F protein. The dN/dS calculated for genotype VII indicate purifying selection, which resulted in a low evolution rate in F gene. The F protein shows a strong negative pressure throughout the length of the protein and no recombination event was determined. Collectively, the results suggest that very similar virulent strains of NDV are involved during current wave of disease outbreak throughout the country. From these results, in conjunction with our recent reports of NDV from Pakistan, it is possible to conclude that emergence of novel group may require revisiting the diagnostics and vaccine control strategies.
KW - Evolutionary analysis
KW - Fusion protein
KW - Newcastle disease
KW - Pakistan
KW - Phylogenetic analysis
U2 - 10.1007/s11262-012-0862-2
DO - 10.1007/s11262-012-0862-2
M3 - Journal article
C2 - 23229206
AN - SCOPUS:84879694912
VL - 46
SP - 309
EP - 315
JO - Virus Genes
JF - Virus Genes
SN - 0920-8569
IS - 2
ER -