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Genetic analysis of peste des petits ruminants virus from Pakistan

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Genetic analysis of peste des petits ruminants virus from Pakistan. / Anees, Muhammad; Shabbir, Muhammad Zubair; Muhammad, Khushi; Nazir, Jawad; Shabbir, Muhammad Abu Bakar; Wensman, Jonas J.; Munir, Muhammad.

In: Veterinary Research, Vol. 9, 60, 28.03.2013.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Anees, M, Shabbir, MZ, Muhammad, K, Nazir, J, Shabbir, MAB, Wensman, JJ & Munir, M 2013, 'Genetic analysis of peste des petits ruminants virus from Pakistan', Veterinary Research, vol. 9, 60. https://doi.org/10.1186/1746-6148-9-60

APA

Anees, M., Shabbir, M. Z., Muhammad, K., Nazir, J., Shabbir, M. A. B., Wensman, J. J., & Munir, M. (2013). Genetic analysis of peste des petits ruminants virus from Pakistan. Veterinary Research, 9, [60]. https://doi.org/10.1186/1746-6148-9-60

Vancouver

Anees M, Shabbir MZ, Muhammad K, Nazir J, Shabbir MAB, Wensman JJ et al. Genetic analysis of peste des petits ruminants virus from Pakistan. Veterinary Research. 2013 Mar 28;9. 60. https://doi.org/10.1186/1746-6148-9-60

Author

Anees, Muhammad ; Shabbir, Muhammad Zubair ; Muhammad, Khushi ; Nazir, Jawad ; Shabbir, Muhammad Abu Bakar ; Wensman, Jonas J. ; Munir, Muhammad. / Genetic analysis of peste des petits ruminants virus from Pakistan. In: Veterinary Research. 2013 ; Vol. 9.

Bibtex

@article{f0f9bad6bc0e4176a5b17e1ba482f141,
title = "Genetic analysis of peste des petits ruminants virus from Pakistan",
abstract = "Background: Peste des petits ruminants (PPR) is an endemic and highly contagious disease in small ruminants of Pakistan. Despite the fact that an effective vaccine is available, outbreaks are regularly occurring in the country. Thus so far, the diagnosis has primarily been made based on clinical outcome or serology. This study was carried out to characterize PPRV from an emerging wave of outbreaks from Punjab, Pakistan. Results: A total of 32 blood samples from five different flocks were tested with real-time PCR for the presence of PPRV genome. The samples detected positive in real-time PCR (n = 17) were subjected to conventional PCR for the amplification of the nucleoprotein (N) gene. Phylogenetic analysis of the sequenced N genes (n = 8) indicated the grouping of all the sequences in lineage IV along with PPRV strains from Asian and Middle East. However, interestingly sequences were divided into two groups. One group of viruses (n = 7) clustered with previously characterized Pakistani isolates whereas one strain of PPRV was distinct and clustered with Saudi Arabian and Iranian strains of PPRV.Conclusions: Results demonstrated in this study expanded the information on the genetic nature of different PPRV population circulating in small ruminants. Such information is essential to understand genetic nature of PPRV strains throughout the country. Proper understanding of these viruses will help to devise control strategies in PPRV endemic countries such as Pakistan.",
author = "Muhammad Anees and Shabbir, {Muhammad Zubair} and Khushi Muhammad and Jawad Nazir and Shabbir, {Muhammad Abu Bakar} and Wensman, {Jonas J.} and Muhammad Munir",
year = "2013",
month = mar,
day = "28",
doi = "10.1186/1746-6148-9-60",
language = "English",
volume = "9",
journal = "Veterinary Research",
issn = "1559-8519",
publisher = "BioMed Central",

}

RIS

TY - JOUR

T1 - Genetic analysis of peste des petits ruminants virus from Pakistan

AU - Anees, Muhammad

AU - Shabbir, Muhammad Zubair

AU - Muhammad, Khushi

AU - Nazir, Jawad

AU - Shabbir, Muhammad Abu Bakar

AU - Wensman, Jonas J.

AU - Munir, Muhammad

PY - 2013/3/28

Y1 - 2013/3/28

N2 - Background: Peste des petits ruminants (PPR) is an endemic and highly contagious disease in small ruminants of Pakistan. Despite the fact that an effective vaccine is available, outbreaks are regularly occurring in the country. Thus so far, the diagnosis has primarily been made based on clinical outcome or serology. This study was carried out to characterize PPRV from an emerging wave of outbreaks from Punjab, Pakistan. Results: A total of 32 blood samples from five different flocks were tested with real-time PCR for the presence of PPRV genome. The samples detected positive in real-time PCR (n = 17) were subjected to conventional PCR for the amplification of the nucleoprotein (N) gene. Phylogenetic analysis of the sequenced N genes (n = 8) indicated the grouping of all the sequences in lineage IV along with PPRV strains from Asian and Middle East. However, interestingly sequences were divided into two groups. One group of viruses (n = 7) clustered with previously characterized Pakistani isolates whereas one strain of PPRV was distinct and clustered with Saudi Arabian and Iranian strains of PPRV.Conclusions: Results demonstrated in this study expanded the information on the genetic nature of different PPRV population circulating in small ruminants. Such information is essential to understand genetic nature of PPRV strains throughout the country. Proper understanding of these viruses will help to devise control strategies in PPRV endemic countries such as Pakistan.

AB - Background: Peste des petits ruminants (PPR) is an endemic and highly contagious disease in small ruminants of Pakistan. Despite the fact that an effective vaccine is available, outbreaks are regularly occurring in the country. Thus so far, the diagnosis has primarily been made based on clinical outcome or serology. This study was carried out to characterize PPRV from an emerging wave of outbreaks from Punjab, Pakistan. Results: A total of 32 blood samples from five different flocks were tested with real-time PCR for the presence of PPRV genome. The samples detected positive in real-time PCR (n = 17) were subjected to conventional PCR for the amplification of the nucleoprotein (N) gene. Phylogenetic analysis of the sequenced N genes (n = 8) indicated the grouping of all the sequences in lineage IV along with PPRV strains from Asian and Middle East. However, interestingly sequences were divided into two groups. One group of viruses (n = 7) clustered with previously characterized Pakistani isolates whereas one strain of PPRV was distinct and clustered with Saudi Arabian and Iranian strains of PPRV.Conclusions: Results demonstrated in this study expanded the information on the genetic nature of different PPRV population circulating in small ruminants. Such information is essential to understand genetic nature of PPRV strains throughout the country. Proper understanding of these viruses will help to devise control strategies in PPRV endemic countries such as Pakistan.

U2 - 10.1186/1746-6148-9-60

DO - 10.1186/1746-6148-9-60

M3 - Journal article

C2 - 23537146

AN - SCOPUS:84875325914

VL - 9

JO - Veterinary Research

JF - Veterinary Research

SN - 1559-8519

M1 - 60

ER -