Present study develops a facile, low-temperature and cost-effective route for the synthesis of nitrogen-doped reduced graphene oxide (N-rGO). The synthesized N-rGO was characterized using X-ray diffraction (XRD), micro-Raman and Fourier transform infrared (FTIR) spectroscopies. An electrochemical sensor using N-rGO-modified glassy carbon electrode (GCE) was fabricated for the determination of dopamine (DA), a neurotransmitter. Because the electrochemical determination and quantification of DA play a significant role in medical diagnosis, such as making soft material-based hydrogel for wound healing. Cyclic voltammetry (CV), amperometry, differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS)-based standard techniques were used to evaluate and establish the optimum electrochemical sensing performance, detection limit, steadiness and reliability of N-rGO/GCE sensing system to the DA detection. The DPV measurements resemble a wide linear range from 100 to 3000 μM and demonstrated a limit of detection (LOD) of 57 nM. It is evidently proved that N-rGO/GCE has great potential to be a preferable electrochemical sensing system for DA detection.