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    Rights statement: This is the author’s version of a work that was accepted for publication in Biomaterials Advances. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomaterials Advances, Vol., pages, 2022 DOI:10.1016/j.bioadv.2022.213094

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Instructive electroactive electrospun silk fibroin-based biomaterials for peripheral nerve tissue engineering

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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Instructive electroactive electrospun silk fibroin-based biomaterials for peripheral nerve tissue engineering. / Phamornnak, Chinnawich; Han, Bing; Spencer, Ben et al.

In: Biomaterials Advances, Vol. 141, 213094, 30.10.2022.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Phamornnak, C, Han, B, Spencer, B, Ashton, M, Blanford, C, Hardy, J, Blaker, J & Cartmell, S 2022, 'Instructive electroactive electrospun silk fibroin-based biomaterials for peripheral nerve tissue engineering', Biomaterials Advances, vol. 141, 213094. https://doi.org/10.1016/j.bioadv.2022.213094

APA

Phamornnak, C., Han, B., Spencer, B., Ashton, M., Blanford, C., Hardy, J., Blaker, J., & Cartmell, S. (2022). Instructive electroactive electrospun silk fibroin-based biomaterials for peripheral nerve tissue engineering. Biomaterials Advances, 141, [213094]. https://doi.org/10.1016/j.bioadv.2022.213094

Vancouver

Phamornnak C, Han B, Spencer B, Ashton M, Blanford C, Hardy J et al. Instructive electroactive electrospun silk fibroin-based biomaterials for peripheral nerve tissue engineering. Biomaterials Advances. 2022 Oct 30;141:213094. Epub 2022 Aug 24. doi: 10.1016/j.bioadv.2022.213094

Author

Phamornnak, Chinnawich ; Han, Bing ; Spencer, Ben et al. / Instructive electroactive electrospun silk fibroin-based biomaterials for peripheral nerve tissue engineering. In: Biomaterials Advances. 2022 ; Vol. 141.

Bibtex

@article{25924766c5b44d88b2841769685f8cc6,
title = "Instructive electroactive electrospun silk fibroin-based biomaterials for peripheral nerve tissue engineering",
abstract = "Aligned sub-micron fibres are an outstanding surface for orienting and promoting neurite outgrowth; therefore, attractive features to include in peripheral nerve tissue scaffolds. A new generation of peripheral nerve tissue scaffolds is under development incorporating electroactive materials and electrical regimes as instructive cues in order to facilitate fully functional regeneration. Herein, electroactive fibres composed of silk fibroin (SF) and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) were developed as a novel peripheral nerve tissue scaffold. Mats of SF with sub-micron fibre diameters of 190 ± 50 nm were fabricated by double layer electrospinning with thicknesses of ∼100 μm (∼70-80 μm random fibres and ∼20-30 μm aligned fibres). Electrospun SF mats were modified with interpenetrating polymer networks (IPN) of PEDOT:PSS in various ratios of PSS/EDOT (α) and the polymerisation was assessed by hard X-ray photoelectron spectroscopy (HAXPES). The mechanical properties of electrospun SF and IPNs mats were characterised in the wet state tensile and the electrical properties were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The cytotoxicity and biocompatibility of the optimal IPNs (α = 2.3 and 3.3) mats were ascertained via the growth and neurite extension of mouse neuroblastoma x rat glioma hybrid cells (NG108-15) for 7 days. The longest neurite outgrowth of 300 μm was observed in the parallel direction of fibre alignment on laminin-coated electrospun SF and IPN (α = 2.3) mats which is the material with the lowest electron transfer resistance (R , ca. 330 Ω). These electrically conductive composites with aligned sub-micron fibres exhibit promise for axon guidance and also have the potential to be combined with electrical stimulation treatment as a further step for the effective regeneration of nerves. [Abstract copyright: Copyright {\textcopyright} 2022. Published by Elsevier B.V.]",
author = "Chinnawich Phamornnak and Bing Han and Ben Spencer and Mark Ashton and Chris Blanford and John Hardy and Jonny Blaker and Sarah Cartmell",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Biomaterials Advances. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomaterials Advances, Vol., pages, 2022 DOI: 10.1016/j.bioadv.2022.213094",
year = "2022",
month = oct,
day = "30",
doi = "10.1016/j.bioadv.2022.213094",
language = "English",
volume = "141",
journal = "Biomaterials Advances",
issn = "2772-9508",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Instructive electroactive electrospun silk fibroin-based biomaterials for peripheral nerve tissue engineering

AU - Phamornnak, Chinnawich

AU - Han, Bing

AU - Spencer, Ben

AU - Ashton, Mark

AU - Blanford, Chris

AU - Hardy, John

AU - Blaker, Jonny

AU - Cartmell, Sarah

N1 - This is the author’s version of a work that was accepted for publication in Biomaterials Advances. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biomaterials Advances, Vol., pages, 2022 DOI: 10.1016/j.bioadv.2022.213094

PY - 2022/10/30

Y1 - 2022/10/30

N2 - Aligned sub-micron fibres are an outstanding surface for orienting and promoting neurite outgrowth; therefore, attractive features to include in peripheral nerve tissue scaffolds. A new generation of peripheral nerve tissue scaffolds is under development incorporating electroactive materials and electrical regimes as instructive cues in order to facilitate fully functional regeneration. Herein, electroactive fibres composed of silk fibroin (SF) and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) were developed as a novel peripheral nerve tissue scaffold. Mats of SF with sub-micron fibre diameters of 190 ± 50 nm were fabricated by double layer electrospinning with thicknesses of ∼100 μm (∼70-80 μm random fibres and ∼20-30 μm aligned fibres). Electrospun SF mats were modified with interpenetrating polymer networks (IPN) of PEDOT:PSS in various ratios of PSS/EDOT (α) and the polymerisation was assessed by hard X-ray photoelectron spectroscopy (HAXPES). The mechanical properties of electrospun SF and IPNs mats were characterised in the wet state tensile and the electrical properties were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The cytotoxicity and biocompatibility of the optimal IPNs (α = 2.3 and 3.3) mats were ascertained via the growth and neurite extension of mouse neuroblastoma x rat glioma hybrid cells (NG108-15) for 7 days. The longest neurite outgrowth of 300 μm was observed in the parallel direction of fibre alignment on laminin-coated electrospun SF and IPN (α = 2.3) mats which is the material with the lowest electron transfer resistance (R , ca. 330 Ω). These electrically conductive composites with aligned sub-micron fibres exhibit promise for axon guidance and also have the potential to be combined with electrical stimulation treatment as a further step for the effective regeneration of nerves. [Abstract copyright: Copyright © 2022. Published by Elsevier B.V.]

AB - Aligned sub-micron fibres are an outstanding surface for orienting and promoting neurite outgrowth; therefore, attractive features to include in peripheral nerve tissue scaffolds. A new generation of peripheral nerve tissue scaffolds is under development incorporating electroactive materials and electrical regimes as instructive cues in order to facilitate fully functional regeneration. Herein, electroactive fibres composed of silk fibroin (SF) and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) were developed as a novel peripheral nerve tissue scaffold. Mats of SF with sub-micron fibre diameters of 190 ± 50 nm were fabricated by double layer electrospinning with thicknesses of ∼100 μm (∼70-80 μm random fibres and ∼20-30 μm aligned fibres). Electrospun SF mats were modified with interpenetrating polymer networks (IPN) of PEDOT:PSS in various ratios of PSS/EDOT (α) and the polymerisation was assessed by hard X-ray photoelectron spectroscopy (HAXPES). The mechanical properties of electrospun SF and IPNs mats were characterised in the wet state tensile and the electrical properties were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The cytotoxicity and biocompatibility of the optimal IPNs (α = 2.3 and 3.3) mats were ascertained via the growth and neurite extension of mouse neuroblastoma x rat glioma hybrid cells (NG108-15) for 7 days. The longest neurite outgrowth of 300 μm was observed in the parallel direction of fibre alignment on laminin-coated electrospun SF and IPN (α = 2.3) mats which is the material with the lowest electron transfer resistance (R , ca. 330 Ω). These electrically conductive composites with aligned sub-micron fibres exhibit promise for axon guidance and also have the potential to be combined with electrical stimulation treatment as a further step for the effective regeneration of nerves. [Abstract copyright: Copyright © 2022. Published by Elsevier B.V.]

U2 - 10.1016/j.bioadv.2022.213094

DO - 10.1016/j.bioadv.2022.213094

M3 - Journal article

VL - 141

JO - Biomaterials Advances

JF - Biomaterials Advances

SN - 2772-9508

M1 - 213094

ER -