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Interactions of multiwalled carbon nanotubes with algal cells: quantification of association, visualization of uptake, and measurement of alterations in the composition of cells

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Interactions of multiwalled carbon nanotubes with algal cells : quantification of association, visualization of uptake, and measurement of alterations in the composition of cells. / Rhiem, Stefan; Riding, Matthew J.; Baumgartner, Werner; Martin, Francis L.; Semple, Kirk T.; Jones, Kevin C.; Schäffer, Andreas; Maes, Hanna M.

In: Environmental Pollution, Vol. 196, 01.2015, p. 431-439.

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Rhiem, Stefan ; Riding, Matthew J. ; Baumgartner, Werner ; Martin, Francis L. ; Semple, Kirk T. ; Jones, Kevin C. ; Schäffer, Andreas ; Maes, Hanna M. / Interactions of multiwalled carbon nanotubes with algal cells : quantification of association, visualization of uptake, and measurement of alterations in the composition of cells. In: Environmental Pollution. 2015 ; Vol. 196. pp. 431-439.

Bibtex

@article{abb7be5d57114e26a2dabc86ff0418d5,
title = "Interactions of multiwalled carbon nanotubes with algal cells: quantification of association, visualization of uptake, and measurement of alterations in the composition of cells",
abstract = "Carbon nanotubes (CNTs) are considered promising materials in nanotechnology. We quantified CNT accumulation by the alga Desmodesmus subspicatus. Cells were exposed to radiolabeled CNTs ((14)C-CNTs; 1 mg/L) to determine uptake and association, as well as elimination and dissociation in clear media. Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was used to detect effects of CNTs on algae. CNT-cell interactions were visualized by electron microscopy and related to alterations in their cell composition. A concentration factor of 5000 L/kg dry weight was calculated. Most of the material agglomerated around the cells, but single tubes were detected in the cytoplasm. Computational analyses of the ATR-FTIR data showed that CNT treated algae differed from controls at all sampling times. CNT exposure changed the biochemical composition of cells. The fact that CNTs are bioavailable for algae and that they influence the cell composition is important with regard to environmental risk assessment of this nanomaterial.",
keywords = "Carbon nanotubes, Green algae , Bioavailability, Electron microscopy , FTIR spectroscopy , Nanoparticles",
author = "Stefan Rhiem and Riding, {Matthew J.} and Werner Baumgartner and Martin, {Francis L.} and Semple, {Kirk T.} and Jones, {Kevin C.} and Andreas Sch{\"a}ffer and Maes, {Hanna M.}",
year = "2015",
month = jan,
doi = "10.1016/j.envpol.2014.11.011",
language = "English",
volume = "196",
pages = "431--439",
journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "Elsevier Ltd",

}

RIS

TY - JOUR

T1 - Interactions of multiwalled carbon nanotubes with algal cells

T2 - quantification of association, visualization of uptake, and measurement of alterations in the composition of cells

AU - Rhiem, Stefan

AU - Riding, Matthew J.

AU - Baumgartner, Werner

AU - Martin, Francis L.

AU - Semple, Kirk T.

AU - Jones, Kevin C.

AU - Schäffer, Andreas

AU - Maes, Hanna M.

PY - 2015/1

Y1 - 2015/1

N2 - Carbon nanotubes (CNTs) are considered promising materials in nanotechnology. We quantified CNT accumulation by the alga Desmodesmus subspicatus. Cells were exposed to radiolabeled CNTs ((14)C-CNTs; 1 mg/L) to determine uptake and association, as well as elimination and dissociation in clear media. Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was used to detect effects of CNTs on algae. CNT-cell interactions were visualized by electron microscopy and related to alterations in their cell composition. A concentration factor of 5000 L/kg dry weight was calculated. Most of the material agglomerated around the cells, but single tubes were detected in the cytoplasm. Computational analyses of the ATR-FTIR data showed that CNT treated algae differed from controls at all sampling times. CNT exposure changed the biochemical composition of cells. The fact that CNTs are bioavailable for algae and that they influence the cell composition is important with regard to environmental risk assessment of this nanomaterial.

AB - Carbon nanotubes (CNTs) are considered promising materials in nanotechnology. We quantified CNT accumulation by the alga Desmodesmus subspicatus. Cells were exposed to radiolabeled CNTs ((14)C-CNTs; 1 mg/L) to determine uptake and association, as well as elimination and dissociation in clear media. Attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) was used to detect effects of CNTs on algae. CNT-cell interactions were visualized by electron microscopy and related to alterations in their cell composition. A concentration factor of 5000 L/kg dry weight was calculated. Most of the material agglomerated around the cells, but single tubes were detected in the cytoplasm. Computational analyses of the ATR-FTIR data showed that CNT treated algae differed from controls at all sampling times. CNT exposure changed the biochemical composition of cells. The fact that CNTs are bioavailable for algae and that they influence the cell composition is important with regard to environmental risk assessment of this nanomaterial.

KW - Carbon nanotubes

KW - Green algae

KW - Bioavailability

KW - Electron microscopy

KW - FTIR spectroscopy

KW - Nanoparticles

U2 - 10.1016/j.envpol.2014.11.011

DO - 10.1016/j.envpol.2014.11.011

M3 - Journal article

C2 - 25467692

VL - 196

SP - 431

EP - 439

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

ER -